YY1 Recombinant Rabbit Monoclonal Antibody [SY29-01] - BSA and Azide free
Catalog# HA751847
YY1 Recombinant Rabbit Monoclonal Antibody [SY29-01] - BSA and Azide free
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WB
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IHC-P
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IP
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
YY1 Recombinant Rabbit Monoclonal Antibody [SY29-01] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human YY1 aa 255-304 / 414.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP, IF-Cell
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
HeLa cell lysate, C2C12 cell lysate, F9 cell lysate, PC-12 cell lysate, C6 cell lysate, human colon tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue, HeLa, C2C12, PC-12.
Conjugation
unconjugated
Clone Number
SY29-01
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The YY1 transcription factor, also known as NF-E1 (human) and Delta or UCRBP (mouse) is of interest due to its diverse effects on a wide variety of target genes. YY1 is broadly expressed in a wide range of cell types and contains four C-terminal zinc finger motifs of the Cys-Cys-His-His type and an unusual set of structural motifs at its N-terminal. It binds to downstream elements in several vertebrate ribosomal protein genes, where it apparently acts positively to stimulate transcription and can act either negatively or positively in the context of the immunoglobulin k 3' enhancer and immunoglobulin heavy-chain μE1 site as well as the P5 promoter of the adeno-associated virus. It thus appears that YY1 is a bifunctional protein, capable of functioning as an activator in some transcriptional control elements and a repressor in others.
Background References
1. Guaraldo M et al. Characterization of human mitochondrial ferritin promoter: identification of transcription factors and evidences of epigenetic control. Sci Rep 6:33432 (2016).
2. Landon AL et al. MNKs act as a regulatory switch for eIF4E1 and eIF4E3 driven mRNA translation in DLBCL. Nat Commun 5:5413 (2014).javascript:;
Sequence Similarity
Belongs to the YY transcription factor family.
Post-translational Modification
Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.; Ubiquitinated.
Subcellular Location
Nucleus matrix.
Synonyms
CF1 antibody
Delta antibody
Delta transcription factor antibody
INO80 complex subunit S antibody
INO80S antibody
NF E1 antibody
NF-E1 antibody
NFE1 antibody
OTTHUMP00000197459 antibody
Transcriptional repressor protein YY1 antibody
ExpandImages
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This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Western blot analysis of YY1 on different lysates with Rabbit anti-YY1 antibody (ET1605-40) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: C2C12 cell lysate
Lane 3: F9 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 68 kDa
Exposure time: 1 minute 21 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-40) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa cells labeling YY1 with Rabbit anti-YY1 antibody (ET1605-40) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-YY1 antibody (ET1605-40) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of C2C12 cells labeling YY1 with Rabbit anti-YY1 antibody (ET1605-40) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-YY1 antibody (ET1605-40) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of PC-12 cells labeling YY1 with Rabbit anti-YY1 antibody (ET1605-40) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-YY1 antibody (ET1605-40) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-YY1 antibody (ET1605-40) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-40) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-YY1 antibody (ET1605-40) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-40) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-YY1 antibody (ET1605-40) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-40) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-YY1 antibody (ET1605-40) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-40) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1605-40, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-YY1 antibody (ET1605-40) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-40) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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