NF-M Recombinant Rabbit Monoclonal Antibody [JM11-20] - BSA and Azide free
Catalog# HA751854
NF-M Recombinant Rabbit Monoclonal Antibody [JM11-20] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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Cynomolgus monkey
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unconjugated
Overview
Product Name
NF-M Recombinant Rabbit Monoclonal Antibody [JM11-20] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human NEFM aa 651-692 / 916.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr, IF-Tissue
Target Molecular Weight
Predicted band size: 102 kDa
Positive Control
Mouse cerebellum tissue, rat cerebellum tissue, human cerebellum tissue, 293T cell lysate, Rat brain tissue lysate, Mouse brain tissue lysate.
Conjugation
unconjugated
Clone Number
JM11-20
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Neurofilament-M (NF-M), for neurofilament medium polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease, and Alzheimer's disease.
Background References
1. Li D et al. NEFM DNA methylation correlates with immune infiltration and survival in breast cancer. Clin Epigenetics. 2021 May
2. Sohrabi N et al. Regulatory Role of Insulin on Endogenous L1 ORF1 and NEFM Gene Expression through PI3K Signaling Pathway Specifically in Neuroblastoma Cell Line. Iran J Public Health. 2023 Mar
Sequence Similarity
Belongs to the intermediate filament family.
Post-translational Modification
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.; Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.; Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Subcellular Location
Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament.
Synonyms
150kDa medium antibody
NEF3 antibody
NEFM antibody
Neurofilament 3 antibody
NF160 antibody
NFM antibody
Images
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This data was developed using ET1703-57, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using ET1703-57, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Rat
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using ET1703-57, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NF-M antibody (ET1703-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1703-57, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NF-M antibody (ET1703-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1703-57, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-NF-M antibody (ET1703-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using ET1703-57, the same antibody clone in a different buffer formulation.
Western blot analysis of NF-M on different lysates with Rabbit anti-NF-M antibody (ET1703-57) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (40 µg/Lane)
Lane 3: Rat liver tissue lysate (negative) (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Mouse liver tissue lysate (negative) (40 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-57) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"