CD130 / GP130 Recombinant Rabbit Monoclonal Antibody [PSH22-56] - BSA and Azide free
Catalog# HA751869
CD130 / GP130 Recombinant Rabbit Monoclonal Antibody [PSH22-56] - BSA and Azide free
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WB
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Mouse
-
unconjugated
Overview
Product Name
CD130 / GP130 Recombinant Rabbit Monoclonal Antibody [PSH22-56] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse CD130 / GP130 aa 1-617.
Species Reactivity
Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 102 kDa
Positive Control
NIH/3T3 cell lysate, MEF cell lysate, Mouse thymus tissue lysate, Mouse lung tissue lysate.
Conjugation
unconjugated
Clone Number
PSH22-56
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
Glycoprotein 130 (also known as gp130, IL6ST, IL6R-beta or CD130) is a transmembrane protein which is the founding member of the class of tall cytokine receptors. It forms one subunit of the type I cytokine receptor within the IL-6 receptor family. It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement. As with other type I cytokine receptors, gp130 possesses a WSXWS amino acid motif that ensures correct protein folding and ligand binding. It interacts with Janus kinases to elicit an intracellular signal following receptor interaction with its ligand. Structurally, gp130 is composed of five fibronectin type-III domains and one immunoglobulin-like C2-type (immunoglobulin-like) domain in its extracellular portion.
Background References
1. Zanders L et al. Sepsis induces interleukin 6, gp130/JAK2/STAT3, and muscle wasting. J Cachexia Sarcopenia Muscle. 2022 Feb
2. Zhu X et al. Intermittent Fasting-Induced Orm2 Promotes Adipose Browning via the GP130/IL23R-p38 Cascade. Adv Sci (Weinh). 2024 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD130 antibody
CD130 antigen antibody
CDw130 antibody
gp130 antibody
GP130 RAPS antibody
IL 6R beta antibody
IL-6 receptor subunit beta antibody
IL-6R subunit beta antibody
IL-6R-beta antibody
IL-6RB antibody
ExpandImages
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Western blot analysis of CD130 / GP130 on different lysates with Rabbit anti-CD130 / GP130 antibody (HA751869) at 1/5,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: MEF cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 130 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751869) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD130 / GP130 on different lysates with Rabbit anti-CD130 / GP130 antibody (HA751869) at 1/5,000 dilution.
Lane 1: Mouse thymus tissue lysate
Lane 2: Mouse lung tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 130 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751869) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"