LDHB Recombinant Rabbit Monoclonal Antibody [PSH22-91] - BSA and Azide free
Catalog# HA751880
LDHB Recombinant Rabbit Monoclonal Antibody [PSH22-91] - BSA and Azide free
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WB
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IF-Cell
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FC
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IP
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Human
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Mouse
-
Rat
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Green monkey
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Pig
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HA724336
含抗保成分
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unconjugated
Overview
Product Name
LDHB Recombinant Rabbit Monoclonal Antibody [PSH22-91] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human LDHB aa 1-334.
Species Reactivity
Human, Mouse, Rat, Green monkey, Pig
Validated Applications
WB, IF-Cell, FC, IP
Molecular Weight
Predicted band size: 37 kDa
Positive Control
HEK-293 (Human embryonic kidney cells) cell lysate, Huh7 (Human liver cancer cells) cell lysate, LNCaP (Human prostate cancer cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Mouse brain tissue lysate, Rat heart tissue lysate, Rat kidney tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH22-91
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:20,000-1:50,000
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IF-Cell
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1:500
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.
Background References
1. Fan S et al. LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection. Autophagy. 2021 Sep
2. Hong W et al. PGC-1alpha loss promotes mitochondrial protein lactylation in acetaminophen-induced liver injury via the LDHB-lactate axis. Pharmacol Res. 2024 Jul
Subcellular Location
Cytoplasm, Mitochondrion inner membrane.
Synonyms
Epididymis secretory protein Li 281 antibody
HEL S 281 antibody
L lactate dehydrogenase B chain antibody
L-lactate dehydrogenase B chain antibody
Lactate dehydrogenase H chain antibody
LDH B antibody
LDH H antibody
LDH heart subunit antibody
LDH-B antibody
LDH-H antibody
ExpandImages
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Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA751880) at 1/20,000 dilution.
Lane 1: HEK-293 (Human embryonic kidney cells) cell lysate
Lane 2: Huh7 (Human liver cancer cells) cell lysate
Lane 3: LNCaP (Human prostate cancer cells) cell lysate
Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lane 6: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 21 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751880, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA751880) at 1/20,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Mouse brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 6 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751880, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA751880) at 1/50,000 dilution.
Lane 1: Rat heart tissue lysate
Lane 2: Rat kidney tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Pig brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 4 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751880, 1/50,000 in 5% NFDM/TBST, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA751880, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). -
Application: Flow Cytometry (Intra)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA751880 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. -
Immunoprecipitation (IP)
LDHB was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA751880 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751880 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA751880 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA751880 in HeLa cell lysate
Exposure time: 2 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA751880, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 37 kDa
Observed band size: 37 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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LDHB Recombinant Rabbit Monoclonal Antibody [PSH22-91]
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Conjugate: unconjugated
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