T-bet / Tbx21 Recombinant Rabbit Monoclonal Antibody [PSH23-00] - BSA and Azide free
Catalog# HA751883
T-bet / Tbx21 Recombinant Rabbit Monoclonal Antibody [PSH23-00] - BSA and Azide free
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
T-bet / Tbx21 Recombinant Rabbit Monoclonal Antibody [PSH23-00] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 58 kDa
Conjugation
unconjugated
Clone Number
PSH23-00
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:200
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IF-Tissue
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1:200
Target
Function
T-box transcription factor TBX21, also called T-bet (T-box expressed in T cells), is a protein that in humans is encoded by the TBX21 gene. Though being for long thought of only as a master regulator of type 1 immune response, T-bet has recently been shown to be implicated in development of various immune cell subsets and maintenance of mucosal homeostasis. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNg). Expression of the human ortholog also correlates with IFNg expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.
Background References
1. Yang X et al. TBX21 inhibits colorectal cancer metastasis through ARHGAP29/GSK3beta inhibitory signaling- and MYCT1/ZO-1 signaling-dependent manner. Int J Biol Sci. 2025 Jan
2. Zou Z et al. TBX21 knockdown attenuates neuroinflammation induced by intracerebral hemorrhage via the SIRT1-WDR5-H3K4me3 axis. Brain Res Bull. 2025 Sep
Subcellular Location
Nucleus.
UNIPROT
Synonyms
T bet antibody
T box 21 antibody
T box expressed in T cells antibody
T box protein 21 antibody
T box transcription factor TBX21 antibody
T cell specific T box transcription factor antibody
T cell specific T box transcription factor T bet antibody
T PET antibody
T-box protein 21 antibody
T-box transcription factor TBX21 antibody
ExpandImages
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751883, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751883, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751883, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"