ALDH1A3 Recombinant Rabbit Monoclonal Antibody [PSH23-87] - BSA and Azide free
Catalog# HA751916
ALDH1A3 Recombinant Rabbit Monoclonal Antibody [PSH23-87] - BSA and Azide free
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WB
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IF-Cell
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FC
-
IP
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Human
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unconjugated
Overview
Product Name
ALDH1A3 Recombinant Rabbit Monoclonal Antibody [PSH23-87] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ALDH1A3 aa 1-512.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC, IP
Target Molecular Weight
Predicted band size: 56 kDa
Positive Control
U-87 MG (Human glioblastoma cells) cell lysate, A431 (Human epidermoid carcinoma skin squamous cells) cell lysate.
Conjugation
unconjugated
Clone Number
PSH23-87
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:5,000
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IF-Cell
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1:100
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FC
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1:1,000
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IP
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1-2μg/sample
Target
Function
Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. ALDH1A3 gene has been observed progressively downregulated in Human papillomavirus-positive neoplastic keratinocytes derived from uterine cervical preneoplastic lesions at different levels of malignancy. For this reason, ALDH1A3 is likely to be associated with tumorigenesis and may be a potential prognostic marker for uterine cervical preneoplastic lesions progression.
Background References
1. Li D et al. ALDH1A3 Coordinates Metabolism With Gene Regulation in Pulmonary Arterial Hypertension. Circulation. 2021 May
2. Son J et al. Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of β-cell failure. Nat Commun. 2023 Feb
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
Acetaldehyde dehydrogenase 6 antibody
AL1A3_HUMAN antibody
Aldehyde dehydrogenase 1A3 antibody
Aldehyde dehydrogenase 6 antibody
Aldehyde dehydrogenase family 1 member A3 antibody
Aldh1a3 antibody
ALDH1A6 antibody
ALDH6 antibody
RALDH 3 antibody
Retinaldehyde dehydrogenase 3 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of ALDH1A3 on different lysates with Rabbit anti-ALDH1A3 antibody (HA751916) at 1/5,000 dilution.
Lane 1: U-87 MG (Human glioblastoma cells) cell lysate
Lane 2: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 3: MCF7 (Human breast cancer cells) cell lysate
Lane 4: 293T (Human embryonic kidney cells) cell lysate
Lane 5: Caco-2 (Human colorectal adenocarcinoma cells) cell lysate
Lane 6: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Low expression: MCF7 cells (PMID: 31197235).
Low expression of ALDH1A3 protein in 293T, Caco-2, and HeLa cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751916, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 56 kDa
Observed band size: 50-56 kDa -
Western blot analysis of ALDH1A3 on different lysates with Rabbit anti-ALDH1A3 antibody (HA751916) at 1/5,000 dilution.
Lane 1: His-tagged Human ALDH1A1 recombinant protein
Lane 2: His-tagged Human ALDH1A2 recombinant protein
Lane 3: His-tagged Human ALDH1A3 recombinant protein
Lysates/proteins at 20 ng/Lane.
Exposure time: 7 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751916, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted MW: 56 kDa
Observed MW: 58-61 kDa -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: U-87 MG (Human glioblastoma cells)/MCF7 (Human breast cancer cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA751916, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Low expression: MCF7 cell (PMID: 31197235). -
☑ Relative expression (RE)
Application: Flow Cytometry (Intra)
Species: Human
Sample: U-87 MG (Human glioblastoma cells)/MCF7 (Human breast cancer cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA751916 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Low expression: MCF7 cell (PMID: 31197235). -
Immunoprecipitation (IP)
ALDH1A3 was immunoprecipitated in 0.2 mg U-87 MG (Human glioblastoma cells) cell lysate with HA751916 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751916 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: U-87 MG cell lysate (input)
Lane 2: HA751916 IP in U-87 MG cell lysate
Lane 3: Rabbit IgG instead of HA751916 in U-87 MG cell lysate
Exposure time: 14 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA751916, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 56 kDa
Observed band size: 50-56 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"