RYR1 Recombinant Rabbit Monoclonal Antibody [PSH24-83] - BSA and Azide free
Catalog# HA751959
RYR1 Recombinant Rabbit Monoclonal Antibody [PSH24-83] - BSA and Azide free
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WB
-
IHC-Fr
-
IHC-P
-
IP
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
RYR1 Recombinant Rabbit Monoclonal Antibody [PSH24-83] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse RYR1 aa 1,301-1,450.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-Fr, IHC-P, IP
Target Molecular Weight
Predicted band size: 565 kDa
Positive Control
Mouse skeletal muscle tissue lysate, Mouse cerebellum tissue lysate, Rat skeletal muscle tissue lysate, Rat cerebellum tissue lysate, rat skeletal muscle tissue lysates.
Conjugation
unconjugated
Clone Number
PSH24-83
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:10,000
-
IHC-Fr
-
1:500
-
IHC-P
-
1:200-1:1,000
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IP
-
1-2μg/sample
Target
Function
Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca2+ leaking into the cytoplasm. Can also mediate the release of Ca2+ from intracellular stores in neurons, and may thereby promote prolonged Ca2+ signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.
Background References
1. Ogasawara M et al. Update on RYR1-related myopathies. Curr Opin Neurol. 2024 Oct
2. Joseph TT et al. Propofol binds and inhibits skeletal muscle ryanodine receptor 1. Br J Anaesth. 2024 Nov
Subcellular Location
Sarcoplasmic reticulum membrane.
Synonyms
Arrhythmogenic right ventricular dysplasia 2 antibody
ARVC2 antibody
ARVD2 antibody
Brain ryanodine receptor calcium release channel antibody
Brain type ryanodine receptor antibody
Cardiac muscle ryanodine receptor calcium release channel antibody
Cardiac muscle type ryanodine receptor antibody
CCO antibody
Central core disease of muscle antibody
HBRR antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of RYR1 on different lysates with Rabbit anti-RYR1 antibody (HA751959) at 1/10,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Mouse cerebellum tissue lysate
Lane 4: Rat skeletal muscle tissue lysate
Lane 5: Rat liver tissue lysate
Lane 6: Rat cerebellum tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Negative control: liver (PMID:7876312) .
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751959, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 565 kDa
Observed band size: 500 kDa -
Western blot analysis of RYR1 on rat skeletal muscle tissue lysate with Rabbit anti-RYR1 antibody (HA751959) at 1/10,000 dilution.
Lysates/proteins at 30 µg/Lane.
Exposure time: 2 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751959, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 565 kDa
Observed band size: 500 kDa -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Skeletal muscle
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA751959, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunofluorescence (IHC-Fr)
Species: Rat
Tissue: Skeletal muscle
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA751959, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Skeletal muscle
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751959, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Skeletal muscle
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751959, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Cerebellum
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751959, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751959, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative control: liver (PMID:7876312). -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Skeletal muscle
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751959, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Cerebellum
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751959, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751959, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative control: liver (PMID:7876312). -
Immunoprecipitation (IP)
RYR1 was immunoprecipitated in 0.2 mg mouse skeletal muscle tissue lysate with HA751959 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751959 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse skeletal muscle tissue lysate (input)
Lane 2: HA751959 IP in mouse skeletal muscle tissue lysate
Lane 3: Rabbit IgG instead of HA751959 in mouse skeletal muscle tissue lysate
Exposure time: 20 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA751959, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 565 kDa
Observed band size: 500 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"