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Aggrecan Recombinant Rabbit Monoclonal Antibody [PSH24-95] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751961

Aggrecan Recombinant Rabbit Monoclonal Antibody [PSH24-95] - BSA and Azide free

Application

  • IHC-P

  • IF-Tissue

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Aggrecan Recombinant Rabbit Monoclonal Antibody [PSH24-95] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Aggrecan aa 1-400.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-P, IF-Tissue, IHC-Fr

Target Molecular Weight

Predicted band size: 261 kDa

Conjugation

unconjugated

Clone Number

PSH24-95

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The large chondroitin sulfate proteoglycan, aggrecan, is the predominant proteoglycan present in cartilage. Aggrecan is a member of the chondroitin sulphate proteoglycan family, which also includes versican/PG-M, neurocan and brevican. Aggrecan is a complex multidomain macromolecule that undergoes extensive processing and post-translational modification. Aggrecan in cartilage forms aggregates with hyaluronan and link protein, embedded in a collagen network. Aggrecan accounts for the compressive stiffness and resilience of the hyaline cartilage. Many forms of inflammatory arthritis are shown to be accompanied with aggrecan degradation and loss from the cartilage. Brevican is a brain proteoglycan of the aggrecan/versican/neurocan family. In the adult brain, the brevican core protein undergoes proteolytic cleavage and exists as a full-length form a carboxy-terminal fragment and an amino-terminal fragment.

Background References

1. Watanabe H. Aggrecan and versican: two brothers close or apart. Am J Physiol Cell Physiol. 2022 May

2. Otsuka MY et al. Aggrecan immobilizes to perineuronal nets through hyaluronan-dependent and hyaluronan-independent binding activities. J Biol Chem. 2025 Jun

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: P16112 Human

SwissProt: Q61282 Mouse

SwissProt: P07897 Rat

Synonyms

ACAN antibody

AGC 1 antibody

AGC1 antibody

AGCAN antibody

Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody

A0122) antibody

Aggrecan 1 antibody

Aggrecan core protein antibody

Aggrecan proteoglycan antibody

Aggrecan structural proteoglycan of cartilage antibody

Expand

Images

  • This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Cartilage <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Cartilage
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724490, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature. <br /> <br />Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724490, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

    Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

  • This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Cartilage <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Cartilage
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724490, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature. <br /> <br />Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724490, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

    Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

  • This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Cartilage <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Cartilage
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724490, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature. <br /> <br />Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724490, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

    Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

  • This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Cartilage <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Cartilage
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA724490, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Mouse <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature. <br /> <br />Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IF-tissue)

    Species: Mouse
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA724490, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

    Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

  • This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Rat <br />Tissue: Cartilage <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Cartilage
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA724490, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Rat <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724490" style="font-weight: bold;text-decoration: underline;">HA724490</a>, 1/200, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature. <br /> <br />Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

    ☑ Relative expression (RE)

    This data was developed using HA724490, the same antibody clone in a different buffer formulation.
    Application: Immunofluorescence (IF-tissue)

    Species: Rat
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA724490, 1/200, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

    Negative expression of Aggrecan protein in liver is consistent with the predicted expression pattern.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"