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GPI Recombinant Rabbit Monoclonal Antibody [PSH25-26] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751972

GPI Recombinant Rabbit Monoclonal Antibody [PSH25-26] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

GPI Recombinant Rabbit Monoclonal Antibody [PSH25-26] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P, IP

Target Molecular Weight

Predicted band size: 63 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate, HCT 116 (Human colon cancer cell) cell lysate, PC-3 (Human prostate cancer cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse liver tissue lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH25-26

Product Features

Form

Liquid

Concentration

0.5 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:500-1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Glucose-6-phosphate isomerase (GPI), alternatively known as phosphoglucose isomerase/phosphoglucoisomerase (PGI) or phosphohexose isomerase (PHI), is an enzyme ( EC 5.3.1.9) that in humans is encoded by the GPI gene on chromosome 19. This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P). Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor (AMF) based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia, and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.

Background References

1. Hong Z et al. Esculetin inhibits liver cancer by targeting glucose-6-phosphate isomerase mediated glycolysis. Biomed Pharmacother. 2025 Jul

2. Gruda Sussman R et al. Glucose 6 Phosphate Isomerase Deficiency, a Rare Hemolytic Anemia Misdiagnosed as Hereditary Spherocytosis. J Pediatr Hematol Oncol. 2023 Jan

Subcellular Location

Cytoplasm, Secreted.

UNIPROT

SwissProt: P06744 Human

SwissProt: P06745 Mouse

SwissProt: Q6P6V0 Rat

Synonyms

AMF antibody

Aurocrine motility factor antibody

Autocrine motility factor antibody

DKFZp686C13233 antibody

EC 5.3.1.9 antibody

G6PI_HUMAN antibody

Glucose phosphate isomerase antibody

Glucose-6-phosphate isomerase antibody

GNPI antibody

GPI antibody

Expand

Images

  • Western blot analysis of GPI on different lysates with Rabbit anti-GPI antibody (<a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>) at 1/5,000 dilution. <br /> <br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate <br />Lane 2: HCT 116 (Human colon cancer cell) cell lysate <br />Lane 3: PC-3 (Human prostate cancer cell) cell lysate <br />Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate <br />Lane 5: A549 (Human lung adenocarcinoma cell) cell lysate <br />Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate <br /> <br />Lysates/proteins at 10 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 63 kDa <br />Observed band size: 55 kDa

    Western blot analysis of GPI on different lysates with Rabbit anti-GPI antibody (HA751972) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: HCT 116 (Human colon cancer cell) cell lysate
    Lane 3: PC-3 (Human prostate cancer cell) cell lysate
    Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate
    Lane 5: A549 (Human lung adenocarcinoma cell) cell lysate
    Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA751972, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 63 kDa
    Observed band size: 55 kDa

  • Western blot analysis of GPI on different lysates with Rabbit anti-GPI antibody (<a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>) at 1/5,000 dilution. <br /> <br />Lane 1: Mouse liver tissue lysate <br />Lane 2: Mouse spleen tissue lysate <br />Lane 3: Mouse brain tissue lysate <br />Lane 4: Rat liver tissue lysate <br />Lane 5: Rat spleen tissue lysate <br />Lane 6: Rat brain tissue lysate <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 63 kDa <br />Observed band size: 55 kDa

    Western blot analysis of GPI on different lysates with Rabbit anti-GPI antibody (HA751972) at 1/5,000 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Mouse brain tissue lysate
    Lane 4: Rat liver tissue lysate
    Lane 5: Rat spleen tissue lysate
    Lane 6: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA751972, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 63 kDa
    Observed band size: 55 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Colon <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA751972, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Kindey <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Kindey
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA751972, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Colon <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA751972, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Kindey <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>, 1/2,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Kindey
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA751972, 1/2,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Colon <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Colon
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA751972, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Immunoprecipitation (IP) <br /> <br />GPI was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a> at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. <br /> <br />Lane 1: HeLa cell lysate (input) <br />Lane 2: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a> IP in HeLa cell lysate <br />Lane 3: Rabbit IgG instead of <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a> in HeLa cell lysate <br /> <br />Exposure time: 3 seconds <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Primary dilution: <a href="/products/HA751972" style="font-weight: bold;text-decoration: underline;">HA751972</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), 2 hours at room temperature <br /> <br />Predicted band size: 63 kDa <br />Observed band size: 55 kDa

    Immunoprecipitation (IP)

    GPI was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA751972 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751972 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA751972 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA751972 in HeLa cell lysate

    Exposure time: 3 seconds
    Blocking: 5% NFDM/TBST, 1 hour at room temperature

    Primary dilution: HA751972, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

    Predicted band size: 63 kDa
    Observed band size: 55 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"