Skip to content

GLA Recombinant Rabbit Monoclonal Antibody [PSH25-93] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA752005

GLA Recombinant Rabbit Monoclonal Antibody [PSH25-93] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

GLA Recombinant Rabbit Monoclonal Antibody [PSH25-93] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human GLA aa 1-429.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC, IP

Target Molecular Weight

Predicted band size: 49 kDa

Positive Control

MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate.

Conjugation

unconjugated

Clone Number

PSH25-93

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.

Background References

1. Chen SC. et. al. Crystal Structure of alpha-Galactosidase from Thermus thermophilus: Insight into Hexamer Assembly and Substrate Specificity. J Agric Food Chem. 2020 Jun

2. Fei Y. et. al. Cloning and expression of a novel alpha-galactosidase from Lactobacillus amylolyticus L6 with hydrolytic and transgalactosyl properties. PLoS One. 2020 Jul

Subcellular Location

Lysosome.

UNIPROT

SwissProt: P06280 Human

Synonyms

AGAL_HUMAN antibody

Agalsidase alfa antibody

Alpha D galactosidase A antibody

Alpha D galactoside galactohydrolase 1 antibody

Alpha D galactoside galactohydrolase antibody

Alpha gal A antibody

Alpha galactosidase A antibody

Alpha-D-galactosidase A antibody

Alpha-D-galactoside galactohydrolase antibody

Alpha-galactosidase A antibody

Expand

Images

  • This data was developed using <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of GLA on different lysates with Rabbit anti-GLA antibody (<a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>) at 1/5,000 dilution. <br /> <br />Lane 1: MCF7 (Human breast cancer cell) cell lysate <br />Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate <br />Lane 3: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate <br />Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate <br />Lane 5: Hep G2 (Human hepatocellular carcinoma cell) cell lysate <br />Lane 6: LNCaP (Human prostate cancer cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 49 kDa <br />Observed band size: 49 kDa

    This data was developed using HA724585, the same antibody clone in a different buffer formulation.
    Western blot analysis of GLA on different lysates with Rabbit anti-GLA antibody (HA724585) at 1/5,000 dilution.

    Lane 1: MCF7 (Human breast cancer cell) cell lysate
    Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 3: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
    Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate
    Lane 5: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 6: LNCaP (Human prostate cancer cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724585, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

  • This data was developed using <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: MCF7 (Human breast cancer cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    This data was developed using HA724585, the same antibody clone in a different buffer formulation.
    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: MCF7 (Human breast cancer cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA724585, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

  • This data was developed using <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Kidney<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, 1/200, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724585, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Kidney
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724585, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, the same antibody clone in a different buffer formulation.<br />Application: Immunohistochemistry (IHC-P)<br /><br />Species: Human<br />Tissue: Liver<br />Sample: Paraffin-embedded section<br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.<br /><br />Wash buffer: 1× TBST<br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, 1/200, 1 hour at room temperature.<br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    This data was developed using HA724585, the same antibody clone in a different buffer formulation.
    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724585, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • This data was developed using <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, the same antibody clone in a different buffer formulation.<br />Application: Flow Cytometry (Intra) <br /> <br />Species: Human <br />Sample: MCF7 (Human breast cancer cells) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br />

    This data was developed using HA724585, the same antibody clone in a different buffer formulation.
    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: MCF7 (Human breast cancer cells)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724585 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

  • This data was developed using <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, the same antibody clone in a different buffer formulation.<br />Immunoprecipitation (IP)<br /><br />GLA was immunoprecipitated in 0.2 mg MCF7 (Human breast cancer cell) cell lysate with <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a> at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: MCF7 cell lysate (input)<br />Lane 2: <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a> IP in MCF7 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a> in MCF7 cell lysate<br /><br />Exposure time: 2 seconds<br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br /><br />Primary dilution: <a href="/products/HA724585" style="font-weight: bold;text-decoration: underline;">HA724585</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), 2 hours at room temperature<br /><br />Predicted band size: 49 kDa<br />Observed band size: 49 kDa

    This data was developed using HA724585, the same antibody clone in a different buffer formulation.
    Immunoprecipitation (IP)

    GLA was immunoprecipitated in 0.2 mg MCF7 (Human breast cancer cell) cell lysate with HA724585 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724585 at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: MCF7 cell lysate (input)
    Lane 2: HA724585 IP in MCF7 cell lysate
    Lane 3: Rabbit IgG instead of HA724585 in MCF7 cell lysate

    Exposure time: 2 seconds
    Blocking: 5% NFDM/TBST, 1 hour at room temperature

    Primary dilution: HA724585, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"