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IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752016

IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

IP3 Receptor Recombinant Rabbit Monoclonal Antibody [JA11-35] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human IP3 Receptor aa 2190-2280 / 2758.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IHC-Fr, IF-Tissue

Molecular Weight

Predicted band size: 314 kDa

Positive Control

Rat brain tissue lysate, Mouse brain tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

JA11-35

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:1,000

  • IHC-Fr

  • 1:500

  • IF-Tissue

  • 1:500

Target

Function

Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways.

Background References

1. Cao X. et. al. ORP4L couples IP(3) to ITPR1 in control of endoplasmic reticulum calcium release. FASEB J. 2019 Dec

2. Synofzik M. et. al. De novo ITPR1 variants are a recurrent cause of early-onset ataxia, acting via loss of channel function. Eur J Hum Genet. 2018 Nov

Sequence Similarity

Belongs to the InsP3 receptor family.

Tissue Specificity

Widely expressed.

Post-translational Modification

Phosphorylated on tyrosine residues.; Ubiquitination at multiple lysines targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked (By similarity).; Phosphorylated by cAMP kinase (PKA). Phosphorylation prevents the ligand-induced opening of the calcium channels. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1.; Palmitoylated by ZDHHC6 in immune cells, leading to regulate ITPR1 stability and function.

Subcellular Location

Endoplasmic reticulum membrane, secretory vesicle membrane, perinuclear region.

UNIPROT

SwissProt: Q14643 Human

SwissProt: P11881 Mouse

SwissProt: P29994 Rat

Synonyms

4 antibody

5-trisphosphate receptor antibody

5-trisphosphate receptor type 1 antibody

DKFZp313E1334 antibody

DKFZp313N1434 antibody

inositol 1 4 5 triphosphate receptor type 1 antibody

Inositol 1 4 5 trisphosphate Receptor Type 1 antibody

Inositol 1 antibody

InsP3R1 antibody

IP3 antibody

Expand

Images

  • Western blot analysis of IP3 Receptor on different lysates with Rabbit anti-IP3 Receptor antibody (<a href="/products/HA752016" style="font-weight: bold;text-decoration: underline;">HA752016</a>) at 1/1,000 dilution.<br /><br />Lane 1: Rat brain tissue lysate<br />Lane 2: Mouse brain tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 314 kDa<br />Observed band size: 314 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA752016" style="font-weight: bold;text-decoration: underline;">HA752016</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IP3 Receptor on different lysates with Rabbit anti-IP3 Receptor antibody (HA752016) at 1/1,000 dilution.

    Lane 1: Rat brain tissue lysate
    Lane 2: Mouse brain tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 314 kDa
    Observed band size: 314 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA752016) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-IP3 Receptor antibody (<a href="/products/HA752016" style="font-weight: bold;text-decoration: underline;">HA752016</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA752016" style="font-weight: bold;text-decoration: underline;">HA752016</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-IP3 Receptor antibody (HA752016) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA752016) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-IP3 Receptor antibody (<a href="/products/HA752016" style="font-weight: bold;text-decoration: underline;">HA752016</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA752016" style="font-weight: bold;text-decoration: underline;">HA752016</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-IP3 Receptor antibody (HA752016) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA752016) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-IP3 Receptor antibody (<a href="/products/HA752016" style="font-weight: bold;text-decoration: underline;">HA752016</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA752016" style="font-weight: bold;text-decoration: underline;">HA752016</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-IP3 Receptor antibody (HA752016) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA752016) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Application: IF-Tissue<br /><br />Species: Rat<br /><br />Site: cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Rat

    Site: cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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