CD127 Recombinant Rabbit Monoclonal Antibody [JE37-10] - BSA and Azide free

This data was developed using HA721214, the same antibody clone in a different buffer formulation.
Western blot analysis of CD127 on different lysates with Rabbit anti-CD127 antibody (HA721214) at 1/5,000 dilution.

Lane 1: SiHa (Human cervical cancersSquamous cell) cell lysate
Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 3: Daudi (Human Burkitt's lymphoma cell) cell lysate
Lane 4: Raji (Human Burkitt's lymphoma cell) cell lysate
Lane 5: 786-0 (Human renal clear cell adenocarcinoma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minute; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721214, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 52 kDa
Observed band size: 70 kDa

☑ Knockdown (KD)

This data was developed using HA721214, the same antibody clone in a different buffer formulation.
Western blot analysis of CD127 on different lysates with Rabbit anti-CD127 antibody (HA721214) at 1/1,000 dilution.

Lane 1: ID8-si NT cell lysate
Lane 2: ID8-si CD127 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 52 kDa
Observed band size: 70 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721214) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA721214, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of SiHa cells labeling CD127 with Rabbit anti-CD127 antibody (HA721214) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD127 antibody (HA721214) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

This data was developed using HA721214, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of K-562 cells labeling CD127.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721214, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).