FOXF1 Recombinant Rabbit Monoclonal Antibody [PSH27-47] - BSA and Azide free
Catalog# HA752074
FOXF1 Recombinant Rabbit Monoclonal Antibody [PSH27-47] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
FOXF1 Recombinant Rabbit Monoclonal Antibody [PSH27-47] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, A375 (Human amelanotic melanoma cell) cell lysate, mouse lung tissue lysates.
Conjugation
unconjugated
Clone Number
PSH27-47
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the regulation of pulmonary genes as well as embryonic development.
Background References
1. Bian F et al. Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling. Nat Commun. 2023 May
2. Bian F et al. FOXF1 promotes tumor vessel normalization and prevents lung cancer progression through FZD4. EMBO Mol Med. 2024 May
Subcellular Location
Nucleus.
Synonyms
AI450827 antibody
FKH L5 antibody
FKHL 5 antibody
FKHL5 antibody
Forkhead (Drosophila) like 5 antibody
Forkhead box F1 antibody
Forkhead box protein F1 antibody
Forkhead drosophila homolog like 5 antibody
Forkhead like 5 antibody
Forkhead related activator 1 antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA724698, the same antibody clone in a different buffer formulation.
Western blot analysis of FOXF1 on different lysates with Rabbit anti-FOXF1 antibody (HA724698) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: A375 (Human amelanotic melanoma cell) cell lysate
A375 is a low expression (PMID: 29162563).
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724698, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 40 kDa
Observed band size: 40 kDa -
This data was developed using HA724698, the same antibody clone in a different buffer formulation.
Western blot analysis of FOXF1 on mouse lung tissue lysate with Rabbit anti-FOXF1 antibody (HA724698) at 1/5,000 dilution.
Lysates/proteins at 40 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724698, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 40 kDa
Observed band size: 40 kDa -
This data was developed using HA724698, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724698, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724698, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Heart
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724698, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative expression of FOXF1 protein in heart is consistent with the predicted expression pattern. -
This data was developed using HA724698, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724698, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724698, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Heart
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724698, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Negative expression of FOXF1 protein in heart is consistent with the predicted expression pattern.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"