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SERPING1 Recombinant Rabbit Monoclonal Antibody [PSH28-15] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752101

SERPING1 Recombinant Rabbit Monoclonal Antibody [PSH28-15] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

SERPING1 Recombinant Rabbit Monoclonal Antibody [PSH28-15] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 55 kDa

Positive Control

Hep G2 (Human hepatocellular carcinoma cell) cell lysates, Human serum lysate, Human urine lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Mouse plasma lysate, Rat plasma lysate.

Conjugation

unconjugated

Clone Number

PSH28-15

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB
human
Tested Tested
mouse
Tested Tested
rat
Tested Tested

Product Features

Form

Liquid

Concentration

0.5 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.

Background References

1. Drouet C et al. SERPING1 Variants and C1-INH Biological Function: A Close Relationship With C1-INH-HAE. Front Allergy. 2022 Mar

2. Shen Y et al. WNT inhibitor SP5-mediated SERPING1 suppresses lung adenocarcinoma progression via TSC2/mTOR pathway. Cell Death Dis. 2025 Feb

Subcellular Location

Secreted.

UNIPROT

SwissProt: P05155 Human

SwissProt: P97290 Mouse

SwissProt: Q6P734 Rat

Synonyms

C1 esterase inhibitor antibody

C1 Inh antibody

C1 inhibiting factor antibody

C1 inhibitor antibody

C1-inhibiting factor antibody

C1IN antibody

C1Inh antibody

C1NH antibody

complement component 1 inhibitor antibody

esterase inhibitor antibody

Expand

Images

  • This data was developed using <a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of SERPING1 on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-SERPING1 antibody (<a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>) at 1/2,500 dilution. <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 20 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>, 1/2,500 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 55 kDa <br />Observed band size: 95-105kDa

    This data was developed using HA724756, the same antibody clone in a different buffer formulation.
    Western blot analysis of SERPING1 on Hep G2 (Human hepatocellular carcinoma cell) cell lysate with Rabbit anti-SERPING1 antibody (HA724756) at 1/2,500 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724756, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 55 kDa
    Observed band size: 95-105kDa

  • This data was developed using <a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (<a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>) at 1/2,500 dilution. <br /> <br />Lane 1: Human serum lysate <br />Lane 2: Human urine lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>, 1/2,500 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 55 kDa <br />Observed band size: 95-105 kDa

    This data was developed using HA724756, the same antibody clone in a different buffer formulation.
    Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA724756) at 1/2,500 dilution.

    Lane 1: Human serum lysate
    Lane 2: Human urine lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724756, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 55 kDa
    Observed band size: 95-105 kDa

  • This data was developed using <a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (<a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>) at 1/2,500 dilution. <br /> <br />Lane 1: Mouse liver tissue lysate <br />Lane 2: Rat liver tissue lysate <br /> <br />Lysates/proteins at 30 µg/Lane. <br />Exposure time: 20 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>, 1/2,500 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 55 kDa <br />Observed band size: 60-65 kDa

    This data was developed using HA724756, the same antibody clone in a different buffer formulation.
    Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA724756) at 1/2,500 dilution.

    Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724756, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 55 kDa
    Observed band size: 60-65 kDa

  • This data was developed using <a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (<a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>) at 1/2,500 dilution. <br /> <br />Lane 1: Mouse plasma lysate <br />Lane 2: Rat plasma lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 46 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724756" style="font-weight: bold;text-decoration: underline;">HA724756</a>, 1/2,500 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 55 kDa <br />Observed band size: 95-105 kDa

    This data was developed using HA724756, the same antibody clone in a different buffer formulation.
    Western blot analysis of SERPING1 on different lysates with Rabbit anti-SERPING1 antibody (HA724756) at 1/2,500 dilution.

    Lane 1: Mouse plasma lysate
    Lane 2: Rat plasma lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 46 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724756, 1/2,500 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 55 kDa
    Observed band size: 95-105 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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