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STIM2 Recombinant Rabbit Monoclonal Antibody [PSH28-30] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA752107

STIM2 Recombinant Rabbit Monoclonal Antibody [PSH28-30] - BSA and Azide free

Application

  • WB

  • FC(Intra)

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

STIM2 Recombinant Rabbit Monoclonal Antibody [PSH28-30] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human STIM2 aa 401-746.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, FC(Intra)

Target Molecular Weight

Predicted band size: 84 kDa

Positive Control

HEK-293 (Human embryonic kidney cell) cell lysate, BT-20 (Human breast cancer cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH28-30

Product Features

Form

Liquid

Concentration

0.5 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Stromal interaction molecule 2 (STIM2) is a protein that in humans is encoded by the STIM2 gene. This gene is a member of the stromal interaction molecule (STIM) family which comprises only two members together with its homologue STIM1, and likely arose from a common ancestral gene. They encode type 1 transmembrane proteins that are located in the sarco/endoplasmic reticulum (SR / ER) into the cell. Alternative translation initiation from an AUG and a non-AUG (UUG) start site results in the production of two different STIM2 isoforms.

Background References

1. Chhikara A et al. Activity-dependent localization and dynamics of STIM1 and STIM2 at ER-PM contacts in hippocampal neurons. Cell Rep. 2025 Oct

2. Luo R et al. STIM2 variants regulate Orai1/TRPC1/TRPC4-mediated store-operated Ca(2+) entry and mitochondrial Ca(2+) homeostasis in cardiomyocytes. Cell Calcium. 2024 May

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q9P246 Human

SwissProt: P83093 Mouse

Entrez Gene: 117087 Rat

Synonyms

FLJ39527 antibody

KIAA1482 antibody

STIM 2 antibody

STIM2 antibody

STIM2_HUMAN antibody

Stromal interaction molecule 2 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA724771" style="font-weight: bold;text-decoration: underline;">HA724771</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of STIM2 on different lysates with Rabbit anti-STIM2 antibody (<a href="/products/HA724771" style="font-weight: bold;text-decoration: underline;">HA724771</a>) at 1/2,000 dilution. <br /> <br />Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate <br />Lane 2: BT-20 (Human breast cancer cell) cell lysate <br />Lane 3: MDA-MB-231 (Human breast cancer cell) cell lysate <br />Lane 4: MCF7 (Human breast cancer cell) cell lysate <br />Lane 5: T-47D (Human breast ductal carcinoma cell) cell lysate <br />Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate <br />Lane 7: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate <br />Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1801 <br /> <br />Low expression: T-47D (PMID: 31464639) <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724771" style="font-weight: bold;text-decoration: underline;">HA724771</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 84 kDa <br />Observed band size: 100 kDa

    ☑ Relative expression (RE)

    This data was developed using HA724771, the same antibody clone in a different buffer formulation.
    Western blot analysis of STIM2 on different lysates with Rabbit anti-STIM2 antibody (HA724771) at 1/2,000 dilution.

    Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate
    Lane 2: BT-20 (Human breast cancer cell) cell lysate
    Lane 3: MDA-MB-231 (Human breast cancer cell) cell lysate
    Lane 4: MCF7 (Human breast cancer cell) cell lysate
    Lane 5: T-47D (Human breast ductal carcinoma cell) cell lysate
    Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 7: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
    Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Low expression: T-47D (PMID: 31464639)

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724771, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 84 kDa
    Observed band size: 100 kDa

  • This data was developed using <a href="/products/HA724771" style="font-weight: bold;text-decoration: underline;">HA724771</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of STIM2 on different lysates with Rabbit anti-STIM2 antibody (<a href="/products/HA724771" style="font-weight: bold;text-decoration: underline;">HA724771</a>) at 1/2,000 dilution. <br /> <br />Lane 1: Mouse spleen tissue lysate <br />Lane 2: Mouse brain tissue lysate <br />Lane 3: Rat spleen tissue lysate <br />Lane 4: Rat brain tissue lysate <br /> <br />Lysates/proteins at 30 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724771" style="font-weight: bold;text-decoration: underline;">HA724771</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 84 kDa <br />Observed band size: 100 kDa

    This data was developed using HA724771, the same antibody clone in a different buffer formulation.
    Western blot analysis of STIM2 on different lysates with Rabbit anti-STIM2 antibody (HA724771) at 1/2,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Rat spleen tissue lysate
    Lane 4: Rat brain tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724771, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 84 kDa
    Observed band size: 100 kDa

  • This data was developed using <a href="/products/HA724771" style="font-weight: bold;text-decoration: underline;">HA724771</a>, the same antibody clone in a different buffer formulation.<br />Application: Flow Cytometry (Intra) <br /> <br />Species: Human <br />Sample: HEK-293 (Human embryonic kidney cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. <br />Antibody dilution buffer: 1x PBS. <br /> <br />Primary antibody: <a href="/products/HA724771" style="font-weight: bold;text-decoration: underline;">HA724771</a> (1/200, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature. <br /> <br />

    This data was developed using HA724771, the same antibody clone in a different buffer formulation.
    Application: Flow Cytometry (Intra)

    Species: Human
    Sample: HEK-293 (Human embryonic kidney cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724771 (1/200, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"