MSR1 / CD204 Recombinant Rabbit Monoclonal Antibody [PSH29-03] - BSA and Azide free
Catalog# HA752136
MSR1 / CD204 Recombinant Rabbit Monoclonal Antibody [PSH29-03] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Mouse
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unconjugated
Overview
Product Name
MSR1 / CD204 Recombinant Rabbit Monoclonal Antibody [PSH29-03] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse MRS1 aa 79-458.
Species Reactivity
Mouse
Validated Applications
WB, IF-Cell, IHC-P, FC, IP
Target Molecular Weight
Predicted band size: 50 kDa
Positive Control
RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, RAW264.7 treated with 50nM PMA and 5μg/mL Lipopolysaccharide for 24 hours cell lysate, J774A.1 (Mouse monocyte macrophage) cell lysate.
Conjugation
unconjugated
Clone Number
PSH29-03
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | IP | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Macrophage scavenger receptor 1, also known as MSR1. MSR1 has also been designated CD204 (cluster of differentiation 204). This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. They were thought to be expressed macrophage-specific, but recently shown to be present on different dendritic cells classes, too. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.
Background References
1. Sheng W et al. Role of macrophage scavenger receptor MSR1 in the progression of non-alcoholic steatohepatitis. Front Immunol. 2022 Dec
2. Gudgeon J et al. The role of macrophage scavenger receptor 1 (MSR1) in inflammatory disorders and cancer. Front Immunol. 2022 Oct
Subcellular Location
Membrane.
UNIPROT
Synonyms
CD204 antibody
CD204 antigen antibody
Macrophage acetylated LDL receptor I and II antibody
Macrophage scavenger receptor 1 antibody
Macrophage scavenger receptor type III antibody
Macrophage scavenger receptor types I and II antibody
Msr 1 antibody
MSR1 antibody
phSR1 antibody
phSR2 antibody
ExpandImages
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☑ Cell treatment (CT)
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Western blot analysis of MSR1 / CD204 on different lysates with Rabbit anti-MSR1 / CD204 antibody (HA724831) at 1/5,000 dilution.
Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
Lane 2: RAW264.7 treated with 50nM PMA and 5μg/mL Lipopolysaccharide for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724831, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 50 kDa
Observed band size: 70-90 kDa -
☑ Relative expression (RE)
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Western blot analysis of MSR1 / CD204 on different lysates with Rabbit anti-MSR1 / CD204 antibody (HA724831) at 1/5,000 dilution.
Lane 1: J774A.1 (Mouse monocyte macrophage) cell lysate
Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 3 minutes; ECL: K1802
Negative control: Neuro-2a, NIH/3T3 (PMID: 35937296).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724831, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 50 kDa
Observed band size: 70-90 kDa -
☑ Cell treatment (CT)
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cell) treated with or without 50 nM PMA and 5 μg/mL LPS for 24 hours
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724831, 1/5,000, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
☑ Relative expression (RE)
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: J774A.1 (Mouse monocyte macrophage) / NIH/3T3 (Mouse fibroblast)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724831, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Negative control: NIH/3T3 (PMID: 35937296). -
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Thymus
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724831, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Spleen
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724831, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724831, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: RAW264.7 (Mouse monocytic macrophage leukemia cell)
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724831, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: J774A.1 (Mouse monocyte macrophage)
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724831, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Neuro-2a (Mouse brain neuroblastoma cell)
Sample: Paraffin-embedded section
Negative control: Neuro-2a (PMID: 35937296).
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724831, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts) (Left, negative) / J774A.1 (Mouse monocyte macrophage)
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724831(1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative control: NIH/3T3 (PMID: 35937296). -
☑ Cell treatment (CT)
This data was developed using HA724831, the same antibody clone in a different buffer formulation.
Immunoprecipitation (IP)
MSR1 / CD204 was immunoprecipitated in 0.2 mg RAW264.7 (Mouse monocytic macrophage leukemia cell) treated with 50nM PMA and 5μg/mL LPS for 24 hours cell lysate with HA724831 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724831 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: RAW264.7 treated with 50nM PMA and 5μg/mL LPS for 24 hours cell lysate (input)
Lane 2: HA724831 IP in RAW264.7 treated with 50nM PMA and 5μg/mL LPS for 24 hours cell lysate
Lane 3: Rabbit IgG instead of HA724831 in RAW264.7 treated with 50nM PMA and 5μg/mL LPS for 24 hours cell lysate
Exposure time: 2 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724831, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 50 kDa
Observed band size: 70-90 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"