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HDAC8 Recombinant Rabbit Monoclonal Antibody [SD082-07]

Usd: 385

Specification

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Catalog# ET1612-90

HDAC8 Recombinant Rabbit Monoclonal Antibody [SD082-07]

Application

  • WB

  • FC

  • IP

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HDAC8 Recombinant Rabbit Monoclonal Antibody [SD082-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HDAC8 aa 61-110 / 377.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, FC, IP

Molecular Weight

Predicted band size: 42 kDa

Positive Control

K562 cell lysate, Hela cell lysate, K562.

Conjugation

unconjugated

Clone Number

SD082-07

RRID

AB_2889884

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

Target

Function

Histone deacetylase 8 is an enzyme that in humans is encoded by the HDAC8 gene. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation / deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. Histone deacetylase 8 is involved in skull morphogenesis and metabolic control of the ERR-alpha / PGC1-alpha transcriptional complex. HDAC8 has been linked to number of disease states notably to acute myeloid leukemia and is related to actin cytoskeleton in smooth muscle cells. siRNA targeting HDAC8 showed anticancer effects. Inhibition of HDAC8 induced apoptosis has been observed in T cell lymphomas. In addition the HDAC8 enzyme has been implicated in the pathogenesis of neuroblastoma.

Background References

1. Deng, H. et al. 2016. Protective effect of Ac-SDKP on alveolar epithelial cells through inhibition of EMT via TGF-β1/ROCK1 pathway in silicosis in rat. Toxicol. Appl. Pharmacol.. 294: 1-10.

2. Lv, W. et al. 2014. Histone deacetylase 1 and 3 regulate the mesodermal lineage commitment of mouse embryonic stem cells. PloS one. 9: e113262.

Sequence Similarity

Belongs to the histone deacetylase family. HD type 1 subfamily.

Tissue Specificity

Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.

Post-translational Modification

Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.

Subcellular Location

Nucleus, Cytoplasm.

UNIPROT

SwissProt: Q9BY41 Human

SwissProt: Q8VH37 Mouse

SwissProt: B1WC68 Rat

Synonyms

CDA07 antibody

CDLS5 antibody

HD 8 antibody

HD8 antibody

HDAC 8 antibody

Hdac8 antibody

HDAC8_HUMAN antibody

HDACL 1 antibody

HDACL1 antibody

Histone deacetylase 8 antibody

Expand

Images

  • Western blot analysis of HDAC8 on different lysates with Rabbit anti-HDAC8 antibody (<a href="/products/ET1612-90" style="font-weight: bold;text-decoration: underline;">ET1612-90</a>) at 1/500 dilution.<br /><br />Lane 1: K562 cell lysate<br />Lane 2: Hela cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 1 minute;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-90" style="font-weight: bold;text-decoration: underline;">ET1612-90</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HDAC8 on different lysates with Rabbit anti-HDAC8 antibody (ET1612-90) at 1/500 dilution.

    Lane 1: K562 cell lysate
    Lane 2: Hela cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 1 minute;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HDAC8 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1612-90" style="font-weight: bold;text-decoration: underline;">ET1612-90</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HDAC8 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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metafield image

HDAC8 Recombinant Rabbit Monoclonal Antibody [JJ0845]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated