HIF Prolyl Hydroxylases Rabbit Polyclonal Antibody
Catalog# ER1910-60
HIF Prolyl Hydroxylases Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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Mouse
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Rat
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unconjugated
Overview
Product Name
HIF Prolyl Hydroxylases Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human PH-4 201-300/502
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
55 KDa
Positive Control
Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:500-2000
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
Target
Function
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Background References
暂无
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein.
UNIPROT
Synonyms
EGLN4 antibody
FLJ20262 antibody
HIF prolyl hydroxylase PH4 antibody
HIF-PH4 antibody
HIF-prolyl hydroxylase 4 antibody
HIFPH4 antibody
HPH-4 antibody
Hypoxia inducible factor prolyl 4 hydroxylase antibody
Hypoxia inducible factor prolyl hydroxylase antibody
Hypoxia-inducible factor prolyl hydroxylase 4 antibody
ExpandImages
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Sample:
Muscle (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- HIF Prolyl Hydroxylases (ER1910-60) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD -
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF Prolyl Hydroxylases) Polyclonal Antibody, Unconjugated (ER1910-60) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
HIF Prolyl Hydroxylases Recombinant Rabbit Monoclonal Antibody [JE63-25]
Application: WB,IHC-P,IF-Cell,IP,FC
Reactivity: Human
Conjugate: unconjugated
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