HLA-DR Recombinant Rabbit Monoclonal Antibody [PSH26-51] - BSA and Azide free
Catalog# HA752035
HLA-DR Recombinant Rabbit Monoclonal Antibody [PSH26-51] - BSA and Azide free
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
HLA-DR Recombinant Rabbit Monoclonal Antibody [PSH26-51] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human HLA-DR aa 51-216.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 29 kDa
Positive Control
Raji (Human Burkitt's lymphoma cell) cell lysate, Daudi (Human Burkitt's lymphoma cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate.
Conjugation
unconjugated
Clone Number
PSH26-51
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IF-Cell
-
1:500
-
IHC-P
-
1:9,000
Target
Function
Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II α/β dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (β-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.
Background References
1. Mastropasqua R et al. Corneoscleral limbus in glaucoma patients: in vivo confocal microscopy and immunocytological study. Invest Ophthalmol Vis Sci 56:2050-8 (2015).
2. Wang H et al. CD68(+)HLA-DR(+) M1-like macrophages promote motility of HCC cells via NF-kB/FAK pathway. Cancer Lett 345:91-9 (2014).
Subcellular Location
Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus, Endosome membrane, Lysosome membrane.
UNIPROT
Synonyms
DR alpha chain antibody
DR alpha chain precursor antibody
DRA_HUMAN antibody
DRB1 antibody
DRB4 antibody
Histocompatibility antigen HLA DR alpha antibody
HLA class II histocompatibility antigen antibody
HLA class II histocompatibility antigen DR alpha chain antibody
HLA DR1B antibody
HLA DR3B antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of HLA-DR on different lysates with Rabbit anti-HLA-DR antibody (HA752035) at 1/5,000 dilution.
Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate
Lane 2: Daudi (Human Burkitt's lymphoma cell) cell lysate
Lane 3: Ramos (Human Burkitt's lymphoma cell) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Negative expression of HLA-DR protein in Jurkat cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA752035, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 29 kDa
Observed band size: 30-40 kDa -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: Raji (Human Burkitt's lymphoma cell)/Jurkat (Human T-lymphoblastic cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Negative expression of HLA-DR protein in Jurkat cell is consistent with the predicted expression pattern
Primary antibody: HA752035, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Bladder adenocarcinoma
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752035, 1/9,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752035, 1/9,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA752035, 1/9,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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