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HSD3B1 Recombinant Rabbit Monoclonal Antibody [PSH18-08]

Usd: 385

Specification

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Catalog# HA723971

HSD3B1 Recombinant Rabbit Monoclonal Antibody [PSH18-08]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

HSD3B1 Recombinant Rabbit Monoclonal Antibody [PSH18-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human HSD3B1 aa 1-300.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 42 kDa

Positive Control

Caco-2 cell lysate, Mouse ovary tissue lysate, Rat ovary tissue lysate, mouse adrenal gland tissue, mouse ovary tissue, rat adrenal gland tissue, rat ovary tissue.

Conjugation

unconjugated

Clone Number

PSH18-08

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell
human
Tested Tested
Not Recommended
Not Recommended
Not Recommended
mouse
Tested Tested
Tested Tested
 
 
rat
Tested Tested
Tested Tested
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

Target

Function

HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. While it can carry out the same function as HSD3B2, it localizes primarily to different tissues, such as the placenta and nonsteroidogenic tissues. Its requirement for the production of progesterone by the placenta, which has a vital role in pregnancy, may be one reason why no disease based on mutations in this gene has been identified to date, besides prostate cancer.

Background References

1. Han FF et al. HSD3B1 variant and androgen-deprivation therapy outcome in prostate cancer. Cancer Chemother Pharmacol. 2021 Jan

2. Thomas L et al. Germline HSD3B1 Genetics and Prostate Cancer Outcomes. Urology. 2020 Nov

Subcellular Location

Endoplasmic reticulum membrane, Mitochondrion membrane.

UNIPROT

SwissProt: P14060 Human

SwissProt: P24815 Mouse

SwissProt: P22071 Rat

Synonyms

3 beta and steroid delta isomerase 1 antibody

3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody

3 beta HSD I antibody

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I antibody

3-beta-HSD I antibody

3-beta-hydroxy-5-ene steroid dehydrogenase antibody

3BETAHSD antibody

3BH antibody

3BHS1_HUMAN antibody

3BHSD antibody

Expand

Images

  • Western blot analysis of HSD3B1 on different lysates with Rabbit anti-HSD3B1 antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/5,000 dilution.<br /><br />Lane 1: Caco-2 cell lysate (20 µg/Lane)<br />Lane 2: Caco-2 cell lysate (no heat) (20 µg/Lane)<br />Lane 3: Mouse ovary tissue lysate (20 µg/Lane)<br />Lane 4: Rat ovary tissue lysate (20 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute 16 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HSD3B1 on different lysates with Rabbit anti-HSD3B1 antibody (HA723971) at 1/5,000 dilution.

    Lane 1: Caco-2 cell lysate (20 µg/Lane)
    Lane 2: Caco-2 cell lysate (no heat) (20 µg/Lane)
    Lane 3: Mouse ovary tissue lysate (20 µg/Lane)
    Lane 4: Rat ovary tissue lysate (20 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute 16 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723971) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue with Rabbit anti-HSD3B1 antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue with Rabbit anti-HSD3B1 antibody (HA723971) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723971) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-HSD3B1 antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-HSD3B1 antibody (HA723971) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723971) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue with Rabbit anti-HSD3B1 antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue with Rabbit anti-HSD3B1 antibody (HA723971) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723971) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-HSD3B1 antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723971" style="font-weight: bold;text-decoration: underline;">HA723971</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-HSD3B1 antibody (HA723971) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723971) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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