HSPA1L Recombinant Mouse Monoclonal Antibody [7D3-R]
Catalog# HA601245
HSPA1L Recombinant Mouse Monoclonal Antibody [7D3-R]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
HSPA1L Recombinant Mouse Monoclonal Antibody [7D3-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein with Human HSPA1L aa 335-639 / 641.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 70 kDa
Positive Control
NCI-H1299 cell lysate, HeLa cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HeLa, human testis tissue, mouse epididymis tissue, mouse testis tissue, rat testis tissue.
Conjugation
unconjugated
Clone Number
7D3-R
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
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IHC-P
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1:1,000-1:40,000 (IF-Tissue is not recommended)
Target
Function
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, the transportation of nascent poly-peptide chains across cellular membranes and the regulation of protein folding. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. HSPA1L (heat shock 70 kDa protein 1L), also known as HSP70T, is a 641 amino acid protein that belongs to the HSP 70 family and, like other HSP proteins, mediates protein folding within the cytosol, as well as within other organelles throughout the cell. HSP proteins, such as HSPA1L, together with chaperones, bind extended peptide segments with a net hydrophobic character exposed during protein translation, membrane translocation or after stress-induced damage. HSPA1L has been found be expressed in spermatids and is not induced by heat shock.
Background References
1. Radons J. The human HSP70 family of chaperones: where do we stand Cell Stress Chaperones 21:379-404 (2016).
2. Hasson S A et al. High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy. Nature 504:291-295 (2013).
Subcellular Location
Cytosol, Secreted.
Synonyms
Heat shock 70 kDa protein 1 Hom antibody
Heat shock 70 kDa protein 1 like antibody
Heat shock 70 kDa protein 1-Hom antibody
Heat shock 70 kDa protein 1-like antibody
Heat shock 70 kDa protein 1L antibody
Heat shock 70kD protein like 1 antibody
HS71L_HUMAN antibody
HSP70 1L antibody
HSP70 HOM antibody
HSP70-Hom antibody
ExpandImages
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Western blot analysis of HSPA1L on different lysates with Mouse anti-HSPA1L antibody (HA601245) at 1/2,000 dilution.
Lane 1: NCI-H1299 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (40 µg/Lane)
Lane 4: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-2: 5 minutes; Lane 3-4: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601245) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling HSPA1L with Mouse anti-HSPA1L antibody (HA601245) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSPA1L antibody (HA601245) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-HSPA1L antibody (HA601245) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601245) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Mouse anti-HSPA1L antibody (HA601245) at 1/40,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601245) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-HSPA1L antibody (HA601245) at 1/40,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601245) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-HSPA1L antibody (HA601245) at 1/40,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601245) at 1/40,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
HSPA1L Mouse Monoclonal Antibody [7D1]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
HSPA1L Mouse Monoclonal Antibody [7D3]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
