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Hamartin Recombinant Rabbit Monoclonal Antibody [PSH14-67] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751528

Hamartin Recombinant Rabbit Monoclonal Antibody [PSH14-67] - BSA and Azide free

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Hamartin Recombinant Rabbit Monoclonal Antibody [PSH14-67] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Hamartin aa 651-900.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 130 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, MEF cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH14-67

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:20,000

  • IP

  • 1-2μg/sample

Target

Function

Tuberous sclerosis 1 (TSC1), also known as hamartin, is a protein that in humans is encoded by the TSC1 gene. TSC1 functions as a co-chaperone which inhibits the ATPase activity of the chaperone Hsp90 (heat shock protein-90) and decelerates its chaperone cycle. TSC1 functions as a facilitator of Hsp90 in chaperoning the kinase and non-kinase clients including TSC2, therefore preventing their ubiquitination and degradation in the proteasome. TSC1, TSC2 and TBC1D7 is a multi-protein complex also known as the TSC complex. This complex negatively regulates mTORC1 signaling by functioning as a GTPase-activating protein (GAP) for the small GTPase Rheb, an essential activator of mTORC1. The TSC complex has been implicated as a tumor suppressor.

Background References

1. Li S et al. Hamartin: An Endogenous Neuroprotective Molecule Induced by Hypoxic Preconditioning. Front Genet. 2020 Sep

Subcellular Location

Lysosome membrane, Cytoplasm, cytosol.

UNIPROT

SwissProt: Q92574 Human

SwissProt: Q9EP53 Mouse

SwissProt: Q9Z136 Rat

Synonyms

Hamartin antibody

kiaa0243 antibody

LAM antibody

TSC antibody

Tsc1 antibody

Tsc1 gene antibody

TSC1_HUMAN antibody

Tuberous sclerosis 1 antibody

Tuberous sclerosis 1 protein antibody

tumor suppressor antibody

Images

  • Western blot analysis of Hamartin on different lysates with Rabbit anti-Hamartin antibody (<a href="/products/HA751528" style="font-weight: bold;text-decoration: underline;">HA751528</a>) at 1/20,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: COS-1 cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: MEF cell lysate<br />Lane 6: C2C12 cell lysate<br />Lane 7: PC-12 cell lysate<br />Lane 8: C6 cell lysate<br />Lane 9: Mouse brain tissue lysate<br />Lane 10: Mouse heart tissue lysate<br />Lane 11: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 130 kDa<br />Observed band size: 140 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751528" style="font-weight: bold;text-decoration: underline;">HA751528</a>) at 1/20,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Hamartin on different lysates with Rabbit anti-Hamartin antibody (HA751528) at 1/20,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: COS-1 cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: MEF cell lysate
    Lane 6: C2C12 cell lysate
    Lane 7: PC-12 cell lysate
    Lane 8: C6 cell lysate
    Lane 9: Mouse brain tissue lysate
    Lane 10: Mouse heart tissue lysate
    Lane 11: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 130 kDa
    Observed band size: 140 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751528) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Hamartin was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with <a href="/products/HA751528" style="font-weight: bold;text-decoration: underline;">HA751528</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751528" style="font-weight: bold;text-decoration: underline;">HA751528</a> at 1/20,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: NIH/3T3 cell lysate (input)<br />Lane 2: <a href="/products/HA751528" style="font-weight: bold;text-decoration: underline;">HA751528</a> IP in NIH/3T3 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751528" style="font-weight: bold;text-decoration: underline;">HA751528</a> in NIH/3T3 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute 50 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Hamartin was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA751528 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751528 at 1/20,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: NIH/3T3 cell lysate (input)
    Lane 2: HA751528 IP in NIH/3T3 cell lysate
    Lane 3: Rabbit IgG instead of HA751528 in NIH/3T3 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute 50 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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