Haptoglobin Mouse Monoclonal Antibody [A10-F5]
Catalog# M1510-36
Haptoglobin Mouse Monoclonal Antibody [A10-F5]
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WB
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IHC-P
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ELISA
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Haptoglobin Mouse Monoclonal Antibody [A10-F5]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Native protein.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, ELISA
Molecular Weight
Predicted band size: 45 kDa
Positive Control
Human plasma lysates, human liver tissue, mouse liver tissue, rat liver tissue.
Conjugation
unconjugated
Clone Number
A10-F5
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:4,000
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IHC-P
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1:1,000
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ELISA
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1:1,000
Target
Function
Haptoglobin (abbreviated as Hp) is the protein that in humans is encoded by the HP gene. In blood plasma, haptoglobin binds with high affinity to free hemoglobin released from erythrocytes, and thereby inhibits its deleterious oxidative activity. Hemoglobin that has been released into the blood plasma by damaged red blood cells has harmful effects. The HP gene encodes a preproprotein that is processed to yield both alpha and beta chains, which subsequently combines as a tetramer to produce haptoglobin. Haptoglobin functions to bind the free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. The cellular receptor target of Hp is the monocyte/macrophage scavenger receptor, CD163. Following Hb-Hp binding to CD163, cellular internalization of the complex leads to globin and heme metabolism, which is followed by adaptive changes in antioxidant and iron metabolism pathways and macrophage phenotype polarization.
Background References
1. Abdullah M & Goldmann T Pulmonary haptoglobin (pHp) is part of the surfactant system in the human lung. Diagn Pathol 7:158 (2012).
2. Tomazic PV et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol N/A:N/A (2013).
Sequence Similarity
Belongs to the peptidase S1 family.
Tissue Specificity
Expressed by the liver and secreted in plasma.
Subcellular Location
Secreted.
Synonyms
Binding peptide antibody
BP antibody
Haptoglobin alpha chain antibody
Haptoglobin alpha(1S) beta antibody
Haptoglobin alpha(2FS) beta antibody
Haptoglobin beta chain antibody
Haptoglobin, alpha polypeptide antibody
Haptoglobin, beta polypeptide antibody
HP antibody
HP2 ALPHA2 antibody
ExpandImages
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Western blot analysis of Haptoglobin on human plasma lysates with Mouse anti-Haptoglobin antibody (M1510-36) at 1/4,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-36) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Haptoglobin antibody (M1510-36) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-Haptoglobin antibody (M1510-36) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-Haptoglobin antibody (M1510-36) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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