Histone H4 Mouse Monoclonal Antibody [H2-E7]
Catalog# EM1706-19
Histone H4 Mouse Monoclonal Antibody [H2-E7]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Histone H4 Mouse Monoclonal Antibody [H2-E7]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
11.4 kDa
Positive Control
THP-1 cell lysate, Hela, paraffin-embedded rectum cancer tissues, paraffin-embedded stomach cancer tissues, Raji.
Conjugation
unconjugated
Clone Number
H2-E7
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-cell
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1:200-1:500
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IHC-P
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1:200-1:500
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FC
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1:100-1:200
Target
Function
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.
Background References
1. Quarato G et al. Sequential Engagement of Distinct MLKL Phosphatidylinositol-Binding Sites Executes Necroptosis. Mol Cell N/A:N/A (2016).
2. Sako K et al. Ky-2, a Histone Deacetylase Inhibitor, Enhances High-Salinity Stress Tolerance in Arabidopsis thaliana. Plant Cell Physiol 57:776-83 (2016).
Subcellular Location
Nucleus. Chromosome.
UNIPROT
Synonyms
dJ160A22.1 antibody
dJ160A22.2 antibody
dJ221C16.1 antibody
dJ221C16.9 antibody
FO108 antibody
H4 antibody
H4.k antibody
H4/a antibody
H4/b antibody
H4/c antibody
ExpandImages
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Western blot analysis of Histone H4 on THP-1 cell lysate using anti- Histone H4 antibody at 1/1,000 dilution.
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ICC staining Histone H4 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using anti- Histone H4 antibody.
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Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti- Histone H4 antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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