Biotin Conjugated Human BST2 Recombinant Rabbit Monoclonal Antibody [PSH23-97] - Detector

Sandwich ELISA analysis of Human BST2 matched pair antibodies

Capture: HA725329, Human BST2 Rabbit mAb [PSH23-96]
Detector: HA725330, Human BST2 Rabbit mAb [PSH23-97]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725329) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human BST2 protein (HA211225) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725330, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native BST2 in A431 and HeLa lysis samples.

Capture: HA725329, Human BST2 Rabbit mAb [PSH23-96]
Detector: HA725330, Human BST2 Rabbit mAb [PSH23-97]

The concentrations of BST2 were measured in duplicates, interpolated from the BST2 standard curve and corrected for sample dilution. Undiluted samples are 200ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BST2 concentration was determined to be 2.26 ng/ml in A431 lysis and 3.26 ng/ml in HeLa lysis.