FITC Conjugated Human CD14 Recombinant Rabbit Monoclonal Antibody [SC69-02]
Catalog# HA720194F
FITC Conjugated Human CD14 Recombinant Rabbit Monoclonal Antibody [SC69-02]
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FC
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Human
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FITC
Overview
Product Name
FITC Conjugated Human CD14 Recombinant Rabbit Monoclonal Antibody [SC69-02]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD14 aa 310-335 / 375.
Species Reactivity
Human
Validated Applications
FC
Molecular Weight
Predicted band size: 40 kDa
Positive Control
Human peripheral blood.
Conjugation
FITC
Clone Number
SC69-02
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2℃ to 8℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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FC
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5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Target
Function
Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.
Background References
1. Dutertre CA et al. Deciphering the stromal and hematopoietic cell network of the adventitia from non-aneurysmal and aneurysmal human aorta. PLoS One 9:e89983 (2014).
2. Hsu RY et al. LPS-induced TLR4 signaling in human colorectal cancer cells increases beta1 integrin-mediated cell adhesion and liver metastasis. Cancer Res 71:1989-98 (2011).
Subcellular Location
Cell membrane, Secreted, Golgi apparatus, Membrane raft.
UNIPROT
Synonyms
CD 14 antibody
CD_antigen=CD14 antibody
CD14 antibody
CD14 antigen antibody
CD14 molecule antibody
CD14_HUMAN antibody
LPS-R antibody
Mo2 antibody
Monocyte differentiation antigen CD14 antibody
Monocyte differentiation antigen CD14 urinary form antibody
ExpandImages
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Flow cytometric analysis of human peripheral blood labelling Human CD14 (HA720194F, FITC).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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