Biotin Conjugated Human CNTF Recombinant Rabbit Monoclonal Antibody [PSH11-82] - Detector
Overview
Product Name
Biotin Conjugated Human CNTF Recombinant Rabbit Monoclonal Antibody [PSH11-82] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CNTF aa 1-200.
Species Reactivity
Human, Rat
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human CNTF protein (HA210944).
Conjugation
Biotin
Clone Number
PSH11-82
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-81] to Human CNTF antibody (Capture) (HA723384) and Recombinant Human CNTF protein (HA210944) as the standard. The reference range value is 31.2-2,000 pg/ml.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a polypeptide hormone and neurotrophic factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations including astrocytes. It is a hypothalamic neuropeptide that is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also cotranscribed with the upstream ZFP91 gene. Cotranscription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor. CNTF has also been shown to be expressed by cells on the bone surface, and to reduce the activity of bone-forming cells (osteoblasts).
Background References
1. Quan H et al. Ciliary neurotrophic factor stimulates stem/progenitor Leydig cell proliferation but inhibits differentiation into its lineage in rats. Andrology. 2023 Oct
2. Do Rhee K et al. Ciliary neurotrophic factor-mediated neuroprotection involves enhanced glycolysis and anabolism in degenerating mouse retinas. Nat Commun. 2022 Nov
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
Ciliary neurotrophic factor antibody
CNTF antibody
CNTF_HUMAN antibody
HCNTF antibody
Images
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Sandwich ELISA analysis of human CNTF matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723384) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CNTF protein (HA210944) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723386B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native CNTF in rat hypothalamus extract samples based on a 1000 µg/ml extract load.
Interpolated concentration of native CNTF was measured in duplicate at different sample concentrations and interpolated from the CNTF standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CNTF concentration was determined to be 120 pg/mL in rat hypothalamus tissue extract. -
Interpolated concentrations of spiked CNTF in cell culture media samples.
The concentrations of CNTF were measured in duplicates, interpolated from the CNTF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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