HRP Conjugated Human CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH12-66] - Detector
Overview
Product Name
HRP Conjugated Human CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH12-66] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CSF-1-R aa 20-517 (HA211149).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human CSF-1-R protein (HA211149).
Conjugation
HRP
Clone Number
PSH12-66
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-66] to Human CSF-1-R antibody (Detector) (HA723474) and recombinant Human CSF-1-R protein (HA211149) as the standard. The reference range value is 1,370-111,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115 (Cluster of Differentiation 115), is a cell-surface protein encoded by the human CSF1R gene (known also as c-FMS). CSF1R is a receptor that can be activated by two ligands: colony stimulating factor 1 (CSF-1) and interleukin-34 (IL-34). CSF1R is highly expressed in myeloid cells, and CSF1R signaling is necessary for the survival, proliferation, and differentiation of many myeloid cell types in vivo and in vitro. CSF1R signaling is involved in many diseases and is targeted in therapies for cancer, neurodegeneration, and inflammatory bone diseases.
Background References
1. Chadarevian JP et al. Engineering an inhibitor-resistant human CSF1R variant for microglia replacement. J Exp Med. 2023 Mar
2. Adams RC et al. CSF1R inhibition promotes neuroinflammation and behavioral deficits during graft-versus-host disease in mice. Blood. 2024 Mar
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
C FMS antibody
CD 115 antibody
CD115 antibody
CD115 antigen antibody
CFMS antibody
Colony stimulating factor 1 receptor antibody
Colony stimulating factor I receptor antibody
CSF 1 R antibody
CSF 1R antibody
CSF-1 receptor antibody
ExpandImages
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![Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies<br /><br />Capture: <a href="/products/HA723477" style="font-weight: bold;text-decoration: underline;">HA723477</a>, Human CSF-1-R Rabbit mAb [PSH12-68]<br />Detector: <a href="/products/HA723474" style="font-weight: bold;text-decoration: underline;">HA723474</a>, Human CSF-1-R Rabbit mAb [PSH12-66]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723477" style="font-weight: bold;text-decoration: underline;">HA723477</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (<a href="/products/HA211149" style="font-weight: bold;text-decoration: underline;">HA211149</a>) starting from 111,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723474" style="font-weight: bold;text-decoration: underline;">HA723474</a>, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723478H_1.jpg?v=20260604174808)
Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies
Capture: HA723477, Human CSF-1-R Rabbit mAb [PSH12-68]
Detector: HA723474, Human CSF-1-R Rabbit mAb [PSH12-66]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723477) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 111,000 pg/ml to 0 pg/ml and detect antibody (HA723474, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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