Human Clusterin Recombinant Rabbit Monoclonal Antibody [PSH15-56] - BSA and Azide free (Detector)
Overview
Product Name
Human Clusterin Recombinant Rabbit Monoclonal Antibody [PSH15-56] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Clusterin aa 23-449 (HA210955).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human Clusterin protein (HA210955).
Conjugation
unconjugated
Clone Number
PSH15-56
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH15-56] to Human Clusterin antibody (Detector) (HA725092) and Recombinant Human Clusterin protein (HA210955) as the standard. The reference range value is 1.370-1,000 ng/mL.
Target
Function
Clusterin (apolipoprotein J) is a 75-80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. In humans, clusterin is encoded by the CLU gene on chromosome 8. CLU is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging.
Background References
1. De Miguel Z et al. Exercise plasma boosts memory and dampens brain inflammation via clusterin. Nature. 2021 Dec
2. Yuste-Checa P et al. The chaperone Clusterin in neurodegeneration-friend or foe? Bioessays. 2022 Jul
Subcellular Location
Nucleus, Cytoplasm, Mitochondrion membrane, cytosol, Microsome, Endoplasmic reticulum, Mitochondrion, perinuclear region, Cytoplasmic vesicle, secretory vesicle, chromaffin granule.
UNIPROT
Synonyms
40 antibody
AAG 4 antibody
AAG4 antibody
Aging associated protein 4 antibody
Aging-associated gene 4 protein antibody
AI893575 antibody
APO J antibody
Apo-J antibody
APOJ antibody
ApoJalpha antibody
ExpandImages
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![Sandwich ELISA analysis of Human Clusterin matched pair antibodies<br /><br />Capture: <a href="/products/HA725091" style="font-weight: bold;text-decoration: underline;">HA725091</a>, Human Clusterin Rabbit mAb [PSH15-55]<br />Detector: <a href="/products/HA725092" style="font-weight: bold;text-decoration: underline;">HA725092</a>, Human Clusterin Rabbit mAb [PSH15-56]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725091" style="font-weight: bold;text-decoration: underline;">HA725091</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Clusterin protein (<a href="/products/HA210955" style="font-weight: bold;text-decoration: underline;">HA210955</a>) starting from 1000,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725092" style="font-weight: bold;text-decoration: underline;">HA725092</a>, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725092_1.png?v=20260509231246)
Sandwich ELISA analysis of Human Clusterin matched pair antibodies
Capture: HA725091, Human Clusterin Rabbit mAb [PSH15-55]
Detector: HA725092, Human Clusterin Rabbit mAb [PSH15-56]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725091) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Clusterin protein (HA210955) starting from 1000,000 pg/ml to 0 pg/ml and detect antibody (HA725092, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Human Clusterin Recombinant Rabbit Monoclonal Antibody [PSH15-55] - BSA and Azide free (Capture)
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