Human Endoglin / CD105 Recombinant Rabbit Monoclonal Antibody [PSH14-59] - BSA and Azide free (Detector)

Sandwich ELISA analysis of Human Endoglin / CD105 matched pair antibodies

Capture: HA723654, Human Endoglin / CD105 Rabbit mAb [PSH14-58]
Detector: HA723655, Human Endoglin / CD105 Rabbit mAb [PSH14-59]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723654) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Endoglin / CD105 protein (HA211006) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723655, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Endoglin / CD105 matched pair antibodies

Capture: HA723657, Human Endoglin / CD105 Rabbit mAb [PSH14-60]
Detector: HA723655, Human Endoglin / CD105 Rabbit mAb [PSH14-59]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723657) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Endoglin / CD105 protein (HA211006) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723655, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Endoglin / CD105 in Human serum and Jurkat supernatant.

Capture: HA723654, Human Endoglin / CD105 Rabbit mAb [PSH14-58]
Detector: HA723655, Human Endoglin / CD105 Rabbit mAb [PSH14-59]

Interpolated concentration of native Endoglin / CD105 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Endoglin / CD105 concentration was determined to be 3975 pg/ml in Human serum,undetectable in Jurkat cell supernatant.

Interpolated concentrations of native Endoglin / CD105 in Human serum and Jurkat cell culture supernatant.

Capture: HA723657, Human Endoglin / CD105 Rabbit mAb [PSH14-60]
Detector: HA723655, Human Endoglin / CD105 Rabbit mAb [PSH14-59]

Interpolated concentration of native Endoglin / CD105 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Endoglin / CD105 concentration was determined to be 3082pg/ml in Human serum,undetectable in Jurkat cell culture supernatant.

Interpolated concentrations of native Endoglin / CD105 in HUVEC, Jurkat and Raji Cell extract samples based on a 1000 µg/ml extract load.

Capture: HA723654, Human Endoglin / CD105 Rabbit mAb [PSH14-58]
Detector: HA723655, Human Endoglin / CD105 Rabbit mAb [PSH14-59]

Interpolated concentration of native Endoglin / CD105 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Endoglin / CD105 concentration was determined to be 3207 pg/ml in HUVEC Cell extract,undetectable in Jurkat and Raji Cell extract.

Interpolated concentrations of native Endoglin / CD105 in HUVEC, Jurkat and Raji Cell extract samples based on a 1000 µg/ml extract load.

Capture: HA723657, Human Endoglin / CD105 Rabbit mAb [PSH14-60]
Detector: HA723655, Human Endoglin / CD105 Rabbit mAb [PSH14-59]

Interpolated concentration of native Endoglin / CD105 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Endoglin / CD105 concentration was determined to be 3462 pg/ml in HUVEC Cell extract,undetectable in Jurkat and Raji Cell extract.

Interpolated concentrations of spiked ENG in cell culture media samples.

Capture: HA723654, Human Endoglin / CD105 Rabbit mAb [PSH14-58]
Detector: HA723655, Human Endoglin / CD105 Rabbit mAb [PSH14-59]

The concentrations of Endoglin / CD105 were measured in duplicates, interpolated from the Endoglin / CD105 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Interpolated concentrations of spiked Endoglin / CD105 in cell culture media samples.

Capture: HA723657, Human Endoglin / CD105 Rabbit mAb [PSH14-60]
Detector: HA723655, Human Endoglin / CD105 Rabbit mAb [PSH14-59]

The concentrations of Endoglin / CD105 were measured in duplicates, interpolated from the Endoglin / CD105 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).