Biotin Conjugated Human FABP4 Recombinant Rabbit Monoclonal Antibody [PSH25-38] - Detector

Sandwich ELISA analysis of Human FABP4 matched pair antibodies

Capture: HA725472, Human FABP4 Rabbit mAb [PSH25-37]
Detector: HA725473, Human FABP4 Rabbit mAb [PSH25-38]

Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725472) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FABP4 protein (HA211489) starting from 500 pg/ml to 0 pg/ml and detect antibody (HA725473, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native FABP4 in human serum samples.

Capture: HA725472, Human FABP4 Rabbit mAb [PSH25-37]
Detector: HA725473, Human FABP4 Rabbit mAb [PSH25-38]

The concentrations of FABP4 were measured in duplicates, interpolated from the FABP4 standard curve and corrected for sample dilution. Undiluted samples are human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP4 concentration was determined to be 4,381 pg/ml in human serum.