HRP Conjugated Human Fetuin A Recombinant Rabbit Monoclonal Antibody [PSH19-13] - Detector
Overview
Product Name
HRP Conjugated Human Fetuin A Recombinant Rabbit Monoclonal Antibody [PSH19-13] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Fetuin A aa 19-367 (HA211146).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Fetuin A protein (HA211146).
Conjugation
HRP
Clone Number
PSH19-13
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-12] to Human Fetuin A antibody (Capture) (HA724046) and Recombinant Human Fetuin A protein (HA211146) as the standard. The reference range value is 78.1-5,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
alpha-2-HS-glycoprotein (AHSG, Alpha-2-Heremans-Schmid Glycoprotein) also known as fetuin-A is a protein that in humans is encoded by the AHSG gene. Fetuin-A belongs to the fetuin class of plasma binding proteins and is more abundant in fetal than adult blood. Alpha2-HS glycoprotein, a glycoprotein present in the serum, is synthesized by hepatocytes and adipocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. The choroid plexus is an established extrahepatic expression site. The mature circulating AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. Multiple post-translational modifications have been reported. Thus AHSG is a secreted partially phosphorylated glycoprotein with complex proteolytic processing that circulates in blood and extracellular fluids. In the test tube AHSG can bind multiple ligands and therefore has been claimed to be involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Most of these functions await confirmation in vivo.
Background References
1. Zhao P et al. Fetuin-A alleviates neuroinflammation against traumatic brain injury-induced microglial necroptosis by regulating Nrf-2/HO-1 pathway. J Neuroinflammation. 2022 Nov
2. Chekol Abebe E et al. Role of Fetuin-A in the Pathogenesis of Psoriasis and Its Potential Clinical Applications. Clin Cosmet Investig Dermatol. 2022 Apr
Subcellular Location
Secreted.
UNIPROT
Synonyms
59 kDa bone sialic acid containing protein antibody
A2HS antibody
Aa2 066 antibody
AHS antibody
Ahsg antibody
Alpha 2 HS glycoprotein antibody
Alpha 2 HS glycoprotein chain A antibody
Alpha 2 HS glycoprotein chain B antibody
Alpha 2 Z globulin antibody
Alpha 2HS glycoprotein antibody
ExpandImages
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![Sandwich ELISA analysis of Human Fetuin A matched pair antibodies<br /><br />Capture: <a href="/products/HA724046" style="font-weight: bold;text-decoration: underline;">HA724046</a>, Human Fetuin A Rabbit mAb [PSH19-12]<br />Detector: <a href="/products/HA724047" style="font-weight: bold;text-decoration: underline;">HA724047</a>, Human Fetuin A Rabbit mAb [PSH19-13]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA724046" style="font-weight: bold;text-decoration: underline;">HA724046</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fetuin A protein (<a href="/products/HA211146" style="font-weight: bold;text-decoration: underline;">HA211146</a>) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA724047" style="font-weight: bold;text-decoration: underline;">HA724047</a>, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA724047H_1.jpg?v=20260824110419)
Sandwich ELISA analysis of Human Fetuin A matched pair antibodies
Capture: HA724046, Human Fetuin A Rabbit mAb [PSH19-12]
Detector: HA724047, Human Fetuin A Rabbit mAb [PSH19-13]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724046) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fetuin A protein (HA211146) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724047, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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