Biotin Conjugated Human G-CSF Recombinant Rabbit Monoclonal Antibody [PSH13-13] - Detector

Sandwich ELISA analysis of Human G-CSF matched pair antibodies

Capture: HA725001, Human G-CSF Rabbit mAb [PSH13-12]
Detector: HA725002, Human G-CSF Rabbit mAb [PSH13-13]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725001) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/mlovernight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human G-CSF protein (HA210840) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA725002, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked G-CSF in cell culture media samples.

Capture: HA725001, Human G-CSF Rabbit mAb [PSH13-12]
Detector: HA725002, Human G-CSF Rabbit mAb [PSH13-13]

The concentrations of G-CSF were measured in duplicates, interpolated from the G-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).