Biotin Conjugated Human IFN beta Recombinant Rabbit Monoclonal Antibody [PSH17-30] - Detector
Overview
Product Name
Biotin Conjugated Human IFN beta Recombinant Rabbit Monoclonal Antibody [PSH17-30] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IFN beta aa 22-187 (HA211523).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human IFN beta protein (HA211523).
Conjugation
Biotin
Clone Number
PSH17-30
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH17-29] to Human IFN beta antibody (Capture) (HA725052) and Recombinant Human IFN beta protein (HA211523) as the standard. The reference range value is 7.8-1,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli. Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins. Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways. Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor. Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons: acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss. IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth.
Background References
1. Ma X et al. Targeting TCF19 sensitizes MSI endometrial cancer to anti-PD-1 therapy by alleviating CD8(+) T cell exhaustion via TRIM14-IFN-beta axis. Cell Rep. 2023 Aug
2. Kalkar P et al. IFN-beta mediates the anti-osteoclastic effect of bisphosphonates and dexamethasone. Front Pharmacol. 2022 Oct
Subcellular Location
Secreted.
UNIPROT
Synonyms
beta-interferon antibody
Fibroblast interferon antibody
IFB antibody
IFF antibody
IFN beta antibody
IFN-beta antibody
IFNB 1 antibody
IFNB antibody
IFNB_HUMAN antibody
IFNB1 antibody
ExpandImages
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![Sandwich ELISA analysis of Human IFN beta matched pair antibodies<br /><br />Capture: <a href="/products/HA725052" style="font-weight: bold;text-decoration: underline;">HA725052</a>, Human IFN beta Rabbit mAb [PSH17-29]<br />Detector: <a href="/products/HA725053" style="font-weight: bold;text-decoration: underline;">HA725053</a>, Human IFN beta Rabbit mAb [PSH17-30]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725052" style="font-weight: bold;text-decoration: underline;">HA725052</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IFN beta protein (<a href="/products/HA211523" style="font-weight: bold;text-decoration: underline;">HA211523</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725053" style="font-weight: bold;text-decoration: underline;">HA725053</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725054B_1.png?v=20260604174731)
Sandwich ELISA analysis of Human IFN beta matched pair antibodies
Capture: HA725052, Human IFN beta Rabbit mAb [PSH17-29]
Detector: HA725053, Human IFN beta Rabbit mAb [PSH17-30]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725052) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IFN beta protein (HA211523) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725053, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native IFN-β in A549 cell culture supernatant untreated or treated with poly I:C for 24h.<br /><br />Capture: <a href="/products/HA725052" style="font-weight: bold;text-decoration: underline;">HA725052</a>, Human IFN beta Rabbit mAb [PSH17-29]<br />Detector: <a href="/products/HA725053" style="font-weight: bold;text-decoration: underline;">HA725053</a>, Human IFN beta Rabbit mAb [PSH17-30]<br /><br />Interpolated concentration of native IFN-β was measured in duplicate at different sample concentrations and interpolated from the IFN-β standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IFN-β concentration was determined to be 98.9 pg/ml in A549 treated cell culture supernatant, undetectable in A549 untreated cell culture supernatant.](https://storage.huabio.cn/huabio/productImg/HA725054B_2.png?v=20260604174731)
Interpolated concentrations of native IFN-β in A549 cell culture supernatant untreated or treated with poly I:C for 24h.
Capture: HA725052, Human IFN beta Rabbit mAb [PSH17-29]
Detector: HA725053, Human IFN beta Rabbit mAb [PSH17-30]
Interpolated concentration of native IFN-β was measured in duplicate at different sample concentrations and interpolated from the IFN-β standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IFN-β concentration was determined to be 98.9 pg/ml in A549 treated cell culture supernatant, undetectable in A549 untreated cell culture supernatant.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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