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Human IL-6 Mouse Monoclonal Antibody [A6D2]

Usd: 350

Specification

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Catalog# HA601050

Human IL-6 Mouse Monoclonal Antibody [A6D2]

Application

  • WB

  • ELISA

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Human IL-6 Mouse Monoclonal Antibody [A6D2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human IL-6 aa 1-212/212.

Species Reactivity

Human

Validated Applications

WB, ELISA

Molecular Weight

Predicted band size: 24 kDa

Positive Control

Recombinant human IL-6 protein.

Conjugation

unconjugated

Clone Number

A6D2

RRID

AB_3071773

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • ELISA

  • 1:5,000

Target

Function

Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF-alpha and IL-1 and its activation of IL-1ra and IL-10. There is some early evidence that IL-6 can be used as an inflammatory marker for severe COVID-19 infection with poor prognosis, in the context of the wider coronavirus pandemic. IL-6 is secreted by macrophages in response to specific microbial molecules, referred to as pathogen-associated molecular patterns (PAMPs). These PAMPs bind to an important group of detection molecules of the innate immune system, called pattern recognition receptors (PRRs), including Toll-like receptors (TLRs). These are present on the cell surface and intracellular compartments and induce intracellular signaling cascades that give rise to inflammatory cytokine production. IL-6 is an important mediator of fever and of the acute phase response. IL-6 stimulates the inflammatory and auto-immune processes in many diseases such as multiple sclerosis, neuromyelitis optica spectrum disorder (NMOSD), diabetes, atherosclerosis, depression, Alzheimer's disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behçet's disease, rheumatoid arthritis, and intracerebral hemorrhage.

Background References

1. Ibrahim ML. et. al. Expression profiles and function of IL6 in polymorphonuclear myeloid-derived suppressor cells. Cancer Immunol Immunother. 2020 Nov

2. Galoian K. et. al. Analysis of IL6-protein complexes in chondrosarcoma. Biomed Rep. 2018 Jan

Subcellular Location

Secreted.

UNIPROT

SwissProt: P05231 Human

Synonyms

Interleukin BSF 2 antibody

B cell differentiation factor antibody

B cell stimulatory factor 2 antibody

B-cell stimulatory factor 2 antibody

BSF 2 antibody

BSF-2 antibody

BSF2 antibody

CDF antibody

CTL differentiation factor antibody

Cytotoxic T cell differentiation factor antibody

Expand

Images

  • Western blot analysis of Human IL-6 on recombinant protein with Mouse anti-Human IL-6 antibody (<a href="/products/HA601050" style="font-weight: bold;text-decoration: underline;">HA601050</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 50 ng/Lane.<br /><br />Exposure time: 30 seconds;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601050" style="font-weight: bold;text-decoration: underline;">HA601050</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Human IL-6 on recombinant protein with Mouse anti-Human IL-6 antibody (HA601050) at 1/500 dilution.

    Lysates/proteins at 50 ng/Lane.

    Exposure time: 30 seconds;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601050) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Human IL-6 Antibody (<a href="/products/HA601050" style="font-weight: bold;text-decoration: underline;">HA601050</a>) in indirect ELISA.<br /><br />Indirect ELISA analysis of Human IL-6 was performed by coating wells of a 96-well plate with 50 µl per well of Human IL-6 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse Human IL-6 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Human IL-6 Antibody (HA601050) in indirect ELISA.

    Indirect ELISA analysis of Human IL-6 was performed by coating wells of a 96-well plate with 50 µl per well of Human IL-6 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse Human IL-6 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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