Human/Monkey ACE Recombinant Rabbit Monoclonal Antibody [PSH22-82] - BSA and Azide free (Detector)
Catalog# HA724327
Human/Monkey ACE Recombinant Rabbit Monoclonal Antibody [PSH22-82] - BSA and Azide free (Detector)
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ELISA(Det)
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Human
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Cynomolgus monkey
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78.1-20,000 pg/mL
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7.62
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unconjugated
Overview
Product Name
Human/Monkey ACE Recombinant Rabbit Monoclonal Antibody [PSH22-82] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ACE aa 30-1256 (HA211209).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human ACE protein (HA211209).
Conjugation
unconjugated
Clone Number
PSH22-82
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-81] to Human/Monkey ACE (Capture) (HA724326) and Recombinant Human ACE protein (HA211209) as the standard. The reference range value is 78.1-20,000 pg/mL.
Target
Function
Angiotensin-converting enzyme (EC 3.4.15.1), or ACE, is a central component of the renin–angiotensin system (RAS), which controls blood pressure by regulating the volume of fluids in the body. It converts the hormone angiotensin I to the active vasoconstrictor angiotensin II. Therefore, ACE indirectly increases blood pressure by causing blood vessels to constrict. ACE inhibitors are widely used as pharmaceutical drugs for treatment of cardiovascular diseases. Other lesser known functions of ACE are degradation of bradykinin, substance P and amyloid beta-protein. ACE hydrolyzes peptides by the removal of a dipeptide from the C-terminus. Likewise it converts the inactive decapeptide angiotensin I to the octapeptide angiotensin II by removing the dipeptide His-Leu. Angiotensin II is a potent vasoconstrictor in a substrate concentration-dependent manner. Angiotensin II binds to the type 1 angiotensin II receptor (AT1), which sets off a number of actions that result in vasoconstriction and therefore increased blood pressure. ACE is also part of the kinin-kallikrein system where it degrades bradykinin, a potent vasodilator, and other vasoactive peptides. Kininase II is the same as angiotensin-converting enzyme. Thus, the same enzyme (ACE) that generates a vasoconstrictor (ANG II) also disposes of vasodilators (bradykinin).
Background References
1. Álvarez-Zaballos S et al. Angiotensin-Converting Enzyme and Heart Failure. Front Biosci (Landmark Ed). 2023 Jul
2. Khurana V et al. Angiotensin converting enzyme (ACE). Clin Chim Acta. 2022 Jan
Subcellular Location
Cell membrane, Cytoplasm; Secreted.
UNIPROT
Synonyms
ACE 1 antibody
ACE antibody
ACE T antibody
ACE_HUMAN antibody
ACE1 antibody
Angiotensin converting enzyme somatic isoform antibody
Angiotensin converting enzyme testis specific isoform antibody
Angiotensin I converting enzyme 1 antibody
Angiotensin I converting enzyme antibody
Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
ExpandImages
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Sandwich ELISA analysis of Human ACE matched pair antibodies
Capture: HA724326, Human/Monkey ACE Rabbit mAb [PSH22-81]
Detector: HA724327, Human/Monkey ACE Rabbit mAb [PSH22-82]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724326) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ACE protein (HA211209) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA724327, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native ACE in Human serum.
Capture: HA724326, Human/Monkey ACE Rabbit mAb [PSH22-81]
Detector: HA724327, Human/Monkey ACE Rabbit mAb [PSH22-82]
Interpolated concentration of native ACE was measured in duplicate at different sample concentrations and interpolated from the ACE standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 224,145 pg/ml in human serum. -
Interpolated concentrations of native ACE in Monkey serum.
Capture: HA724326, Human/Monkey ACE Rabbit mAb [PSH22-81]
Detector: HA724327, Human/Monkey ACE Rabbit mAb [PSH22-82]
Interpolated concentration of native ACE was measured in duplicate at different sample concentrations and interpolated from the ACE standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 100,598 pg/ml in monkey serum. -
Interpolated concentrations of spiked ACE in cell culture media samples.
Capture: HA724326, Human/Monkey ACE Rabbit mAb [PSH22-81]
Detector: HA724327, Human/Monkey ACE Rabbit mAb [PSH22-82]
The concentrations of ACE were measured in duplicates, interpolated from the ACE standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
ACE Recombinant Rabbit Monoclonal Antibody [JM59-32]
Application: WB,IHC-P,FC,mIHC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Biotin Conjugated Human/Monkey ACE Recombinant Rabbit Monoclonal Antibody [PSH22-82] - Detector
Application: ELISA(Det),ELISA
Reactivity: Human,Cynomolgus monkey
Conjugate: Biotin
Human/Monkey ACE Recombinant Rabbit Monoclonal Antibody [PSH22-81] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Cynomolgus monkey
Conjugate: unconjugated
