HRP Conjugated Human Neuropilin-1 Recombinant Rabbit Monoclonal Antibody [PSH14-74] - Detector
Overview
Product Name
HRP Conjugated Human Neuropilin-1 Recombinant Rabbit Monoclonal Antibody [PSH14-74] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Neuropilin-1 aa 22-644 (HA211017).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Neuropilin-1 protein (HA211017).
Conjugation
HRP
Clone Number
PSH14-74
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-73] to Human Neuropilin-1 antibody (Capture) (HA725112) and Recombinant Human Neuropilin-1 protein (HA211017) as the standard. The reference range value is 976.6-125,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. This protein has also been determined to act as a co-receptor for SARS-CoV-2 (which causes COVID-19) to infect host cells. Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.
Background References
1. Cantuti-Castelvetri L et al. Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity. Science. 2020 Nov
2. Mayi BS et al. The role of Neuropilin-1 in COVID-19. PLoS Pathog. 2021 Jan
Subcellular Location
Mitochondrion membrane, Cell membrane, Cytoplasm; Secreted.
UNIPROT
Synonyms
A5 protein antibody
BDCA4 antibody
BLOOD DENDRITIC CELL ANTIGEN 4 antibody
CD304 antibody
Neuropilin-1 antibody
Neuropilin1 antibody
NP1 antibody
NPN1 antibody
NRP 1 antibody
NRP antibody
ExpandImages
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![Sandwich ELISA analysis of Human Neuropilin-1 matched pair antibodies<br /><br />Capture: <a href="/products/HA725112" style="font-weight: bold;text-decoration: underline;">HA725112</a>, Human Neuropilin-1 Rabbit mAb [PSH14-73]<br />Detector: <a href="/products/HA725113" style="font-weight: bold;text-decoration: underline;">HA725113</a>, Human Neuropilin-1 Rabbit mAb [PSH14-74]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA725112" style="font-weight: bold;text-decoration: underline;">HA725112</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropilin-1 protein (<a href="/products/HA211017" style="font-weight: bold;text-decoration: underline;">HA211017</a>) starting from 125,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725113" style="font-weight: bold;text-decoration: underline;">HA725113</a>, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725114H_1.png?v=20260604174801)
Sandwich ELISA analysis of Human Neuropilin-1 matched pair antibodies
Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73]
Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725112) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropilin-1 protein (HA211017) starting from 125,000 pg/ml to 0 pg/ml and detect antibody (HA725113, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Neuropilin-1 in HT-29 and SK-BR-3 extract samples based on a 1,000 µg/ml extract load.<br /><br />Capture: <a href="/products/HA725112" style="font-weight: bold;text-decoration: underline;">HA725112</a>, Human Neuropilin-1 Rabbit mAb [PSH14-73]<br />Detector: <a href="/products/HA725113" style="font-weight: bold;text-decoration: underline;">HA725113</a>, Human Neuropilin-1 Rabbit mAb [PSH14-74]<br /><br />The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. Undiluted samples are HT-29 extract 50% and SK-BR-3 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 7,020 pg/ml in HT-29 extract and undetectable in SK-BR-3 extract.](https://storage.huabio.cn/huabio/productImg/HA725114H_2.png?v=20260604174801)
Interpolated concentrations of native Neuropilin-1 in HT-29 and SK-BR-3 extract samples based on a 1,000 µg/ml extract load.
Capture: HA725112, Human Neuropilin-1 Rabbit mAb [PSH14-73]
Detector: HA725113, Human Neuropilin-1 Rabbit mAb [PSH14-74]
The concentrations of Neuropilin-1 were measured in duplicates, interpolated from the Neuropilin-1 standard curve and corrected for sample dilution. Undiluted samples are HT-29 extract 50% and SK-BR-3 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuropilin-1 concentration was determined to be 7,020 pg/ml in HT-29 extract and undetectable in SK-BR-3 extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human Neuropilin-1 Recombinant Rabbit Monoclonal Antibody [PSH14-74] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
