HRP Conjugated Human Poliovirus Receptor/PVR Recombinant Rabbit Monoclonal Antibody [PSH12-55] - Detector
Overview
Product Name
HRP Conjugated Human Poliovirus Receptor/PVR Recombinant Rabbit Monoclonal Antibody [PSH12-55] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CD155 / PVR aa 21-343 (HA210957).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Poliovirus Receptor/PVR protein (HA210957).
Conjugation
HRP
Clone Number
PSH12-55
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-54] to Human Poliovirus Receptor/PVR antibody (Capture) (HA723464) and Recombinant Human Poliovirus Receptor/PVR protein (HA210957) as the standard. The reference range value is 312.5-40,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
CD155 (cluster of differentiation 155), also known as the poliovirus receptor, is a protein that in humans is encoded by the PVR gene. It is a transmembrane protein that is involved in forming junctions between neighboring cells. It is also the molecule that poliovirus uses to enter cells. The gene is specific to the primates. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Its normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.
Background References
1. Lee BH et al. PVR (CD155) Expression as a Potential Prognostic Marker in Multiple Myeloma. Biomedicines. 2022 May
2. Murakami K et al. The Nectin family ligands, PVRL2 and PVR, in cancer immunology and immunotherapy. Front Immunol. 2024 Aug
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
CD155 antibody
CD155 antigen antibody
FLJ25946 antibody
HVED antibody
mE4 antibody
NECL 5 antibody
Necl-5 antibody
Necl5 antibody
Nectin like 5 antibody
Nectin like protein 5 antibody
ExpandImages
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![Sandwich ELISA analysis of Human Poliovirus Receptor/PVR matched pair antibodies<br /><br />Capture: <a href="/products/HA723464" style="font-weight: bold;text-decoration: underline;">HA723464</a>, Human Poliovirus Receptor/PVR Rabbit mAb [PSH12-54]<br />Detector: <a href="/products/HA723465" style="font-weight: bold;text-decoration: underline;">HA723465</a>, Human Poliovirus Receptor/PVR Rabbit mAb [PSH12-55]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723464" style="font-weight: bold;text-decoration: underline;">HA723464</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Poliovirus Receptor/PVRn protein (<a href="/products/HA210957" style="font-weight: bold;text-decoration: underline;">HA210957</a>) starting from 40,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723465" style="font-weight: bold;text-decoration: underline;">HA723465</a>, HRP, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723466H_1.jpg?v=20260604174808)
Sandwich ELISA analysis of Human Poliovirus Receptor/PVR matched pair antibodies
Capture: HA723464, Human Poliovirus Receptor/PVR Rabbit mAb [PSH12-54]
Detector: HA723465, Human Poliovirus Receptor/PVR Rabbit mAb [PSH12-55]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723464) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Poliovirus Receptor/PVRn protein (HA210957) starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA723465, HRP, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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