Human SOD2, C-His Tag Protein
Catalog# HA211476
Human SOD2, C-His Tag Protein
Product Information
Product name
Human SOD2, C-His Tag Protein
Catalog No.
HA211476
Predicted molecular mass
24.6 kD
Protein construction description
A DNA sequence encoding the human SOD2 protein (P04179-1) (Lys 25-Lys 222) was expressed with a His tag at the C-terminus.
Accession
P04179-1
Protein construction
Source
E.coli
Bio Activity
Testing in progress.
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
Species
Human
Background
The superoxide dismutase family is composed of three metalloenzymes (SOD-1, SOD-2 and SOD-3) that catalyze the oxido-reduction of reactive oxygen species (ROS) such as superoxide anion. The SOD-2 precursor is a 222 amino acid protein that is encoded by nuclear chromatin, synthesized in the cytosol and imported posttranslationally into the mitochondrial matrix. Unlike SOD-1, which is a homodimeric cytosolic Cu-Zn enzyme, SOD-2 is a homotetrameric manganese enzyme (also known as MnSOD) that functions in the mitochondrion. ROS are implicated in a wide range of degenerative processes, including Alzheimer’s disease, Parkinson’s disease and ischemic heart disease. Homozygous mutant mice, which lack SOD-2, exhibit dilated cardiomyopathy, accumulation of lipid in liver and skeletal muscle, metabolic acidosis, oxidative DNA damage and respiratory chain deficiencies in heart and skeletal muscle. Polymorphisms in the SOD-2 gene have also been implicated in nonfamilial, idiopathic, dilated cardiomyopathy in humans.
Usage guide
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.
Storage
Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
Reconstitution
Reconstitute at 250 μg/ml in sterile water.
Products with the same target and pathway
Human REST, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human Caspase-9/CASP9, Tag Free Protein
Application:
Reactivity: Human
Conjugate:
Human SOD1/Cu-Zn SOD, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
Human KIF5B, N-Twin Strep, C-His Tag Protein
Application:
Reactivity: Human
Conjugate:
