HRP Conjugated Human VCAM-1 Recombinant Rabbit Monoclonal Antibody [PSH12-47] - Detector
Overview
Product Name
HRP Conjugated Human VCAM-1 Recombinant Rabbit Monoclonal Antibody [PSH12-47] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human VCAM-1 aa 25-698 (HA210976).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human VCAM-1 protein (HA210976).
Conjugation
HRP
Clone Number
PSH12-47
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-46] to Human VCAM-1 antibody (Capture) (HA725064) and Recombinant Human VCAM-1 protein (HA210976) as the standard. The reference range value is 1,370-333,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
Vascular cell adhesion protein 1 also known as vascular cell adhesion molecule 1 (VCAM-1) or cluster of differentiation 106 (CD106) is a protein that in humans is encoded by the VCAM1 gene. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development of atherosclerosis and rheumatoid arthritis.
Background References
1. Lau SF et al. The VCAM1-ApoE pathway directs microglial chemotaxis and alleviates Alzheimer\'s disease pathology. Nat Aging. 2023 Oct
2. Zhang S et al. Macrophage-mediated vascular permeability via VLA4/VCAM1 pathway dictates ascites development in ovarian cancer. J Clin Invest. 2021 Feb
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
CD106 antibody
CD106 Antigen antibody
INCAM 100 antibody
INCAM-100 antibody
L1CAM antibody
MGC99561 antibody
V-CAM 1 antibody
Vascular Cell Adhesion Molecule 1 antibody
Vascular cell adhesion protein 1 antibody
VCAM 1 antibody
ExpandImages
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![Sandwich ELISA analysis of Human VCAM-1 matched pair antibodies<br /><br />Capture: <a href="/products/HA725064" style="font-weight: bold;text-decoration: underline;">HA725064</a>, Human VCAM-1 Rabbit mAb [PSH12-46]<br />Detector: <a href="/products/HA725065" style="font-weight: bold;text-decoration: underline;">HA725065</a>, Human VCAM-1 Rabbit mAb [PSH12-47]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725064" style="font-weight: bold;text-decoration: underline;">HA725064</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VCAM-1 protein (<a href="/products/HA210976" style="font-weight: bold;text-decoration: underline;">HA210976</a>) protein starting from 333,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725065" style="font-weight: bold;text-decoration: underline;">HA725065</a>, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725066H_1.jpg?v=20260604174807)
Sandwich ELISA analysis of Human VCAM-1 matched pair antibodies
Capture: HA725064, Human VCAM-1 Rabbit mAb [PSH12-46]
Detector: HA725065, Human VCAM-1 Rabbit mAb [PSH12-47]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725064) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VCAM-1 protein (HA210976) protein starting from 333,000 pg/ml to 0 pg/ml and detect antibody (HA725065, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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