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ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH09-31]

Usd: 385

Specification

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Catalog# HA723089

ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH09-31]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ICAM1 Recombinant Rabbit Monoclonal Antibody [PSH09-31]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ICAM1 aa 1-480.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 58 kDa

Positive Control

HepG2 cell lysate, K-562 cell lysate, Raji cell lysate, Ramos cell lysate, human colon cancer tissue, human kidney tissue, human lymph node tissue.

Conjugation

unconjugated

Clone Number

PSH09-31

Reactivity Data

WB IHC-P IF-Tissue
Human
Tested Tested
Tested Tested
Mouse
Not Recommended
Not Recommended
Rat
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:8,000

  • IF-Tissue

  • 1:1,000

Target

Function

ICAM-1 (Intercellular Adhesion Molecule 1) also known as CD54 (Cluster of Differentiation 54) is a protein that in humans is encoded by the ICAM1 gene. This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor for entry into respiratory epithelium. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor (TNF) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. LFA-1 has also been found in a soluble form, which seems to bind and block ICAM-1.

Background References

1. Bai X et al. CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy. Nat Commun. 2023 Mar

2. Zhou Q et al. Role of ICAM1 in tumor immunity and prognosis of triple-negative breast cancer. Front Immunol. 2023 Aug

Subcellular Location

Membrane.

UNIPROT

SwissProt: P05362 Human

Synonyms

Antigen identified by monoclonal antibody BB2 antibody

BB 2 antibody

BB2 antibody

CD 54 antibody

CD_antigen=CD54 antibody

CD54 antibody

Cell surface glycoprotein P3.58 antibody

Human rhinovirus receptor antibody

ICAM 1 antibody

ICAM-1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: K-562 cell lysate (low expression)<br />Lane 3: Raji cell lysate<br />Lane 4: Ramos cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723089) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: K-562 cell lysate (low expression)
    Lane 3: Raji cell lysate
    Lane 4: Ramos cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 58 kDa
    Observed band size: 110 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723089) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/10,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-ICAM1 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 58 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723089) at 1/10,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-ICAM1 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 58 kDa
    Observed band size: 100 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723089) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody (HA723089) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723089) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody (HA723089) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723089) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723089" style="font-weight: bold;text-decoration: underline;">HA723089</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody (HA723089) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723089) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: colon cancer<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: colon cancer

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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