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ICAM2 Recombinant Rabbit Monoclonal Antibody [PSH25-72] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751991

ICAM2 Recombinant Rabbit Monoclonal Antibody [PSH25-72] - BSA and Azide free

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ICAM2 Recombinant Rabbit Monoclonal Antibody [PSH25-72] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ICAM2 aa 1-248.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 31 kDa

Positive Control

Jurkat (Human T-lymphoblastic cells) cell lysate, EA.hy926 (Human umbilical vein fusion cell) cell lysate.

Conjugation

unconjugated

Clone Number

PSH25-72

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

Target

Function

Intercellular adhesion molecule 2 (ICAM2), also known as CD102 (Cluster of Differentiation 102), is a human gene, and the protein resulting from it. ICAM-2 molecules regulate spermatid adhesion on Sertoli cell on the apical side of the blood-testis barrier (towards the lumen), thus playing a major role in spermatogenesis. This protein may also play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Background References

1. Pan JK et al. ICAM2 initiates trans-blood-CSF barrier migration and stemness properties in leptomeningeal metastasis of triple-negative breast cancer. Oncogene. 2023 Sep

2. Li Z et al. Proteomics uncovers ICAM2 (CD102) as a novel serum biomarker of proliferative lupus nephritis. Lupus Sci Med. 2025 Apr

Subcellular Location

Cell membrane, Cell projection, microvillus.

UNIPROT

SwissProt: P13598 Human

SwissProt: P35330 Mouse

Synonyms

CD102 antibody

CD102 antigen antibody

ICAM 2 antibody

ICAM-2 antibody

Icam2 antibody

ICAM2_HUMAN antibody

Intercellular adhesion molecule 2 antibody

Ly60 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of ICAM2 on different lysates with Rabbit anti-ICAM2 antibody (<a href="/products/HA751991" style="font-weight: bold;text-decoration: underline;">HA751991</a>) at 1/5,000 dilution. <br /> <br />Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate <br />Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate <br />Lane 3: HEK-293 (Human embryonic kidney cell) cell lysate <br />Lane 4: EA.hy926 (Human umbilical vein fusion cell) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1802 <br /> <br />HeLa is a negative control (PMID: 23714211). <br />HEK-293 is a negative control (PMID: 23714211). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA751991" style="font-weight: bold;text-decoration: underline;">HA751991</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 31 kDa <br />Observed band size: 55 kDa

    ☑ Relative expression (RE)

    Western blot analysis of ICAM2 on different lysates with Rabbit anti-ICAM2 antibody (HA751991) at 1/5,000 dilution.

    Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 3: HEK-293 (Human embryonic kidney cell) cell lysate
    Lane 4: EA.hy926 (Human umbilical vein fusion cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1802

    HeLa is a negative control (PMID: 23714211).
    HEK-293 is a negative control (PMID: 23714211).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA751991, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 31 kDa
    Observed band size: 55 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: Jurkat (Human T-lymphoblastic cells)/HEK-293 (Human embryonic kidney cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA751991" style="font-weight: bold;text-decoration: underline;">HA751991</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). <br /> <br />Negative control: HEK-293 (PMID:23714211).

    ☑ Relative expression (RE)

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: Jurkat (Human T-lymphoblastic cells)/HEK-293 (Human embryonic kidney cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA751991, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
    Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Negative control: HEK-293 (PMID:23714211).

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Flow Cytometry<br /><br />Species: Human<br />Sample: HEK-293 (Human embryonic kidney cell)/Jurkat (Human T-lymphoblastic cells)<br /><br />Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. (human)<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/HA751991" style="font-weight: bold;text-decoration: underline;">HA751991</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.<br /><br />Negative control: HEK-293 (PMID: 23714211).

    ☑ Relative expression (RE)

    Application: Flow Cytometry

    Species: Human
    Sample: HEK-293 (Human embryonic kidney cell)/Jurkat (Human T-lymphoblastic cells)

    Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. (human)
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA751991 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

    Negative control: HEK-293 (PMID: 23714211).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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