IGF1R Mouse Monoclonal Antibody [D5-G2]
Catalog# HA600033
IGF1R Mouse Monoclonal Antibody [D5-G2]
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WB
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FC
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Human
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unconjugated
Overview
Product Name
IGF1R Mouse Monoclonal Antibody [D5-G2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human IGF1R aa 600-1000.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 155 kDa
Positive Control
293 cell lysates, SiHa.
Conjugation
unconjugated
Clone Number
D5-G2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500
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FC
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1:50-1:100
Target
Function
Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis.
Background References
1. Kato H. et. al. Role of tyrosine kinase activity in signal transduction by the insulin-like growth factor-I (IGF-I) receptor. Characterization of kinase-deficient IGF-I receptors and the action of an IGF-I-mimetic antibody (alpha IR-3). J. Biol. Chem. 268:2655-2661(1993).
Subcellular Location
Cell membrane, Membrane.
UNIPROT
Synonyms
Insulin-like growth factor 1 receptor beta chain antibody
CD221 antibody
CD221 antigen antibody
IGF 1 receptor antibody
IGF 1R antibody
IGF I receptor antibody
IGF-I receptor antibody
Igf1r antibody
IGF1R_HUMAN antibody
IGFIR antibody
ExpandImages
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Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Flow cytometric analysis of IGF1R was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600033, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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