IL-11RA Recombinant Rabbit Monoclonal Antibody [JE53-88]
Catalog# HA720044
IL-11RA Recombinant Rabbit Monoclonal Antibody [JE53-88]
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WB
-
IF-Cell
-
IHC-P
-
FC
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Human
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Mouse
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unconjugated
Overview
Product Name
IL-11RA Recombinant Rabbit Monoclonal Antibody [JE53-88]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human IL-11RA aa 373-422/422.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 45 kDa
Positive Control
Mouse spleen tissue lysate, mouse testis tissue lysate, mouse thymus tissue lysate, Jurkat cell lysates, human kidney tissue, Hela.
Conjugation
unconjugated
Clone Number
JE53-88
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
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IF-Cell
-
1:50
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IHC-P
-
1:1,000
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FC
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1:500-1:1,000
Target
Function
Interleukin 11 is a stromal cell-derived cytokine that belongs to a family of pleiotropic and redundant cytokines that use the gp130 transducing subunit in their high affinity receptors. This gene encodes the IL-11 receptor, which is a member of the hematopoietic cytokine receptor family. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain. Multiple alternatively spliced transcript variants have been found for this gene.
Background References
1. Korakavi N. et. al. Evolution of the phenotype of craniosynostosis with dental anomalies syndrome and report of IL11RA variant population frequencies in a Crouzon-like autosomal recessive syndrome. Am J Med Genet A. 2019 Apr
2. Brischoux-Boucher E. et. al. IL11RA-related Crouzon-like autosomal recessive craniosynostosis in 10 new patients: Resemblances and differences. Clin Genet. 2018 Oct
Subcellular Location
Membrane, Secreted.
Synonyms
CRSDA antibody
hCG_2011440 antibody
I11RA_HUMAN antibody
IL-11 receptor subunit alpha antibody
IL-11R subunit alpha antibody
IL-11R-alpha antibody
IL-11RA antibody
IL11RA antibody
Interleukin 11 receptor alpha antibody
Interleukin 11 receptor alpha chain antibody
ExpandImages
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Western blot analysis of IL-11RA on different lysates with Rabbit anti-IL-11RA antibody (HA720044) at 1/1,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Mouse thymus tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720044) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of IL-11RA on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720044, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IL-11RA antibody (HA720044) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720044) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of Hela cells labeling IL-11RA with Rabbit anti-IL-11RA antibody (HA720044) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-11RA antibody (HA720044) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of IL-11RA was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720044, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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