Skip to content

IL-18 Recombinant Rabbit Monoclonal Antibody [PSH18-15]

Usd: 389

Specification

Bulk Order Quote | Customization Request

Catalog# HA723978

IL-18 Recombinant Rabbit Monoclonal Antibody [PSH18-15]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IL-18 Recombinant Rabbit Monoclonal Antibody [PSH18-15]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse IL-18 aa 1-192.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 22 kDa

Positive Control

HeLa cell lysate, RAW264.7 cell lysate, J774A.1 cell lysate, C6 cell lysate, Mouse liver tissue lysate, Mouse thymus tissue lysate, Rat liver tissue lysate, mouse liver tissue, mouse thymus tissue, rat thymus tissue.

Conjugation

unconjugated

Clone Number

PSH18-15

Reactivity Data

WB IHC-P IP
Human
Tested Tested
Not Recommended
Mouse
Tested Tested
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:2,000

  • IP

  • 1-2μg/sample

Target

Function

Interleukin-18 (IL-18), also known as interferon-gamma inducing factor is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine. Many cell types, both hematopoietic cells and non-hematopoietic cells, have the potential to produce IL-18. It was first described in 1989 as a factor that induced interferon-γ (IFN-γ) production in mouse spleen cells. Originally, IL-18 production was recognized in Kupffer cells, liver-resident macrophages. However, IL-18 is constitutively expressed in non-hematopoietic cells, such as intestinal epithelial cells, keratinocytes, and endothelial cells. IL-18 can modulate both innate and adaptive immunity and its dysregulation can cause autoimmune or inflammatory diseases. IL-18 belongs to the IL-1 superfamily and is produced mainly by macrophages but also other cell types, stimulates various cell types and has pleiotropic functions. IL-18 is a proinflammatory cytokine that facilitates type 1 responses. Together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 in combination with IL12 acts on CD4, CD8 T cells and NK cells to induce IFNγ production, type II interferon that plays an important role in activating the macrophages or other cells. The combination of this IL-18 and IL-12 has been shown to inhibit IL-4 dependent IgE and IgG1 production and enhance IgG2a production in B cells. Importantly, without IL-12 or IL-15, IL-18 does not induce IFNγ production, but plays an important role in the differentiation of naive T cells into Th2 cells and stimulates mast cells and basophils to produce IL-4, IL-13, and chemical mediators such as histamine.

Background References

1. Jaspers JE et al. IL-18-secreting CAR T cells targeting DLL3 are highly effective in small cell lung cancer models. J Clin Invest. 2023 May

2. Landy E et al. Biological and clinical roles of IL-18 in inflammatory diseases. Nat Rev Rheumatol. 2024 Jan

Subcellular Location

Cytoplasm, cytosol, Secreted.

UNIPROT

SwissProt: Q14116 Human

SwissProt: P70380 Mouse

SwissProt: P97636 Rat

Synonyms

Iboctadekin antibody

IFN gamma inducing factor antibody

IFN-gamma-inducing factor antibody

IGIF antibody

IL 1 gamma antibody

IL 18 antibody

IL 1g antibody

IL-1 gamma antibody

IL-18 antibody

IL1 gamma antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of IL-18 on different lysates with Rabbit anti-IL-18 antibody (<a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Raji cell lysate (negative)<br />Lane 3: RAW264.7 cell lysate<br />Lane 4: J774A.1 cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: Mouse liver tissue lysate<br />Lane 7: Mouse thymus tissue lysate<br />Lane 8: Rat liver tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 22 kDa<br />Observed band size: 22 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of IL-18 on different lysates with Rabbit anti-IL-18 antibody (HA723978) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Raji cell lysate (negative)
    Lane 3: RAW264.7 cell lysate
    Lane 4: J774A.1 cell lysate
    Lane 5: C6 cell lysate
    Lane 6: Mouse liver tissue lysate
    Lane 7: Mouse thymus tissue lysate
    Lane 8: Rat liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723978) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-IL-18 antibody (<a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-IL-18 antibody (HA723978) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723978) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-IL-18 antibody (<a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-IL-18 antibody (HA723978) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723978) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-IL-18 antibody (<a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-IL-18 antibody (HA723978) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723978) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IL-18 was immunoprecipitated from 0.2 mg J774A.1 cell lysate with <a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: J774A.1 cell lysate (input)<br />Lane 2: <a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a> IP in J774A.1 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723978" style="font-weight: bold;text-decoration: underline;">HA723978</a> in J774A.1 cell lysate<br /><br />Blocking/Dilution buffer: primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>)<br />Exposure time: 10 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    IL-18 was immunoprecipitated from 0.2 mg J774A.1 cell lysate with HA723978 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723978 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: J774A.1 cell lysate (input)
    Lane 2: HA723978 IP in J774A.1 cell lysate
    Lane 3: Rabbit IgG instead of HA723978 in J774A.1 cell lysate

    Blocking/Dilution buffer: primary antibody dilution (K1803)
    Exposure time: 10 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit
metafield image

IL-18 Recombinant Rabbit Monoclonal Antibody [PSH18-15] - BSA and Azide free

Application: WB,IHC-P,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

IL-18 Recombinant Rabbit Monoclonal Antibody [PS00-41] - BSA and Azide free

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated

metafield image

Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH01-71] - BSA and Azide free (Capture)

Application: ELISA(Cap)

Reactivity: Human,Dog,Cynomolgus monkey

Conjugate: unconjugated

metafield image

Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH01-70] - BSA and Azide free (Detector)

Application: ELISA(Det)

Reactivity: Human,Dog,Cynomolgus monkey

Conjugate: unconjugated

metafield image

Biotin Conjugated Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH01-70]

Application: ELISA(Det)

Reactivity: Human,Dog,Cynomolgus monkey

Conjugate: Biotin

metafield image

IL-18 Recombinant Rabbit Monoclonal Antibody [PS01-06] - BSA and Azide free

Application: WB,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

IL-18 Recombinant Rabbit Monoclonal Antibody [PS01-06]

Application: WB,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

IL-18 Recombinant Rabbit Monoclonal Antibody [PS00-41]

Application: WB,IHC-P

Reactivity: Human

Conjugate: unconjugated