IL-2 Mouse Monoclonal Antibody [A8B9] - BSA and Azide free
Catalog# HA601016
IL-2 Mouse Monoclonal Antibody [A8B9] - BSA and Azide free
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WB
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ELISA
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Human
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unconjugated
Overview
Product Name
IL-2 Mouse Monoclonal Antibody [A8B9] - BSA and Azide free
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human IL-2 aa 1-153/153.
Species Reactivity
Human
Validated Applications
WB, ELISA
Target Molecular Weight
Predicted band size: 18 kDa
Positive Control
IL-2 recombinant protein.
Conjugation
unconjugated
Clone Number
A8B9
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500
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ELISA
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1:20,000
Target
Function
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15.5–16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. In the thymus, where T cells mature, it prevents autoimmune diseases by promoting the differentiation of certain immature T cells into regulatory T cells, which suppress other T cells that are otherwise primed to attack normal healthy cells in the body. IL-2 enhances activation-induced cell death (AICD). IL-2 also promotes the differentiation of T cells into effector T cells and into memory T cells when the initial T cell is also stimulated by an antigen, thus helping the body fight off infections. Together with other polarizing cytokines, IL-2 stimulates naive CD4+ T cell differentiation into Th1 and Th2 lymphocytes while it impedes differentiation into Th17 and folicular Th lymphocytes. IL-2 increases the cell killing activity of both natural killer cells and cytotoxic T cells. Its expression and secretion is tightly regulated and functions as part of both transient positive and negative feedback loops in mounting and dampening immune responses. Through its role in the development of T cell immunologic memory, which depends upon the expansion of the number and function of antigen-selected T cell clones, it plays a key role in enduring cell-mediated immunity. While the causes of itchiness are poorly understood, some evidence indicates that IL-2 is involved in itchy psoriasis.
Background References
1. Mizui M. Natural and modified IL-2 for the treatment of cancer and autoimmune diseases. Clin Immunol. 2019 Sep
2. Abbas AK. et. al. Revisiting IL-2: Biology and therapeutic prospects. Sci Immunol. 2018 Jul
Subcellular Location
Secreted.
UNIPROT
Synonyms
Aldesleukin antibody
IL 2 antibody
IL-2 antibody
IL2 antibody
IL2_HUMAN antibody
Interleukin 2 antibody
Interleukin-2 antibody
interleukin2 antibody
Involved in regulation of T cell clonal expansion antibody
Lymphokine antibody
ExpandImages
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IL-2 Antibody (HA601016) in indirect ELISA.
Indirect ELISA analysis of IL-2 was performed by coating wells of a 96-well plate with 50 µl per well of IL-2 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse IL-2 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Western blot analysis of IL-2 on IL-2 Recombinant Protein with Mouse anti-IL-2 antibody (HA601016) at 1/1,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time:1 minutes; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601016, 1/1,000 in 5% NFDM/TBST 2h at RT.
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 18 kDa
Observed band size: 18 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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