IL-13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89]
Catalog# ET7106-61
IL-13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89]
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
IL-13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IL13 Receptor alpha 1 aa 1-127 / 427.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 49 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, HepG2, human liver tissue.
Conjugation
unconjugated
Clone Number
JU38-89
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:50-1:200
Target
Function
Interleukin 13 receptor, alpha 1, also known as IL13RA1 and CD213A1 (cluster of differentiation 213A1), is a human gene. Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
Background References
1. Aguirre CA. et. al. Two single nucleotide polymorphisms in IL13 and IL13RA1 from individuals with idiopathic Parkinson\'s disease increase cellular susceptibility to oxidative stress. Brain Behav Immun. 2020 Aug
Sequence Similarity
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Tissue Specificity
Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.
Subcellular Location
Membrane.
UNIPROT
Synonyms
bB128O4.2.1 (interleukin 13 receptor, alpha 1) antibody
Cancer/testis antigen 19 antibody
CD213a1 antibody
CD213A1 Antigen antibody
CT 19 antibody
CT19 antibody
I13R1_HUMAN antibody
IL 13 receptor subunit alpha 1 antibody
IL 13R alpha 1 antibody
IL 13R subunit alpha 1 antibody
ExpandImages
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Western blot analysis of IL-13 Receptor alpha 1 on different lysates with Rabbit anti-IL-13 Receptor alpha 1 antibody (ET7106-61) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 10 second; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-61) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of IL-13 Receptor alpha 1 on different lysates with Rabbit anti-IL-13 Receptor alpha 1 antibody (ET7106-61) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-IL-13 Receptor alpha 1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 60 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-61) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of IL-13 Receptor alpha 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-61, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL-13 Receptor alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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