IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60]
Overview
Product Name
IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IRE1 aa 1-400.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 110 kDa
Positive Control
HEK-293 cell lysate, Neuro-2a cell lysate, C6 cell lysate, COS-1 cell lysate.
Conjugation
unconjugated
Clone Number
PSH10-60
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
| Green Monkey |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
The serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 α (IRE1α) is an enzyme that in humans is encoded by the ERN1 gene. The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals (mainly the unfolded protein response). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References
1. Huang R et al. IRE1 signaling regulates chondrocyte apoptosis and death fate in the osteoarthritis. J Cell Physiol. 2022 Jan
2. Grandjean JMD et al. Pharmacologic IRE1/XBP1s activation confers targeted ER proteostasis reprogramming. Nat Chem Biol. 2020 Oct
Subcellular Location
Endoplasmic reticulum membrane; nucleus.
Synonyms
Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
Endoplasmic reticulum to nucleus signaling 1 antibody
Endoplasmic reticulum-to-nucleus signaling 1 antibody
Endoribonuclease antibody
ER to nucleus signaling 1 antibody
ERN 1 antibody
Ern1 antibody
ERN1_HUMAN antibody
hIRE 1p antibody
hIRE1p antibody
ExpandImages
-
Western blot analysis of IRE1 on different lysates with Rabbit anti-IRE1 antibody (HA723225) at 1/5,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: C6 cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723225) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Quercetin ameliorates renal injury in hyperuricemic rats via modulating ER stress pathways
Journal: Frontiers In Pharmacology
DOI: 10.3389/fphar.2025.1660599
IF: 4.8
Application: WB
Reactivity: Rat
Publish date: 2025 Sept
Products with the same target and pathway
IRE1 Mouse Monoclonal Antibody [C9-F2]
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Reactivity: Human
Conjugate: unconjugated
Phospho-IRE1 (S724) Recombinant Rabbit Monoclonal Antibody [PSH03-35] - BSA and Azide free
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse
Conjugate: unconjugated
IRE1 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
IRE1 Recombinant Rabbit Monoclonal Antibody [PSH10-60] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
