IRF5 Recombinant Rabbit Monoclonal Antibody [SD203-07]
Catalog# ET1612-15
IRF5 Recombinant Rabbit Monoclonal Antibody [SD203-07]
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WB
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
IRF5 Recombinant Rabbit Monoclonal Antibody [SD203-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IRF5 aa 1-200.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Tissue, IHC-P, IP
Molecular Weight
Predicted band size: 56 kDa
Positive Control
THP-1 cell lysate, U-937 cell lysate, A20 cell lysate, Rat spleen tissue lysate, mouse spleen tissue, rat spleen tissue, human spleen tissue.
Conjugation
unconjugated
Clone Number
SD203-07
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
Target
Function
Interferon regulatory factor 5 (IRF-5), belongs to the IRF family of DNA-binding factors, which includes IRF-1, IRF-2, IRF-3, IRF-4, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP). The IRF family regulate both interferon and interferon-inducible genes. IRF-5, like IRF-3 and IRF-7, is a direct transducer of virus-mediated signaling and plays a role in the expression of multiple cytokines/chemokines. Although IRF-5 is a direct target of p53, its cell cycle regulatory and proapoptotic effects are p53 independent.
Background References
1. O\'Carroll C et al. Identification of a unique hybrid macrophage-polarization state following recovery from lipopolysaccharide tolerance. J Immunol 192:427-36 (2014).
2. Chionh YT et al. Protease-activated receptor 1 suppresses Helicobacter pylori gastritis via the inhibition of macrophage cytokine secretion and interferon regulatory factor 5. Mucosal Immunol 8(1):1399:1412 (2014).
Sequence Similarity
Belongs to the IRF family.
Post-translational Modification
Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.; 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
Interferon regulatory factor 5 antibody
Interferon regulatory factor 5 bone marrow variant antibody
IRF 5 antibody
IRF-5 antibody
Irf5 antibody
IRF5_HUMAN antibody
SLEB10 antibody
Images
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Western blot analysis of IRF5 on different lysates with Rabbit anti-IRF5 antibody (ET1612-15) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate (30 µg/Lane)
Lane 2: U-937 cell lysate (30 µg/Lane)
Lane 3: A20 cell lysate (30 µg/Lane)
Lane 4: Rat spleen tissue lysate (30 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-IRF5 antibody (ET1612-15) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-IRF5 antibody (ET1612-15) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IRF5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-15, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
IRF5 Recombinant Rabbit Monoclonal Antibody [SN201-05] - BSA and Azide free
Application: WB,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
