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ITPR2 Rabbit Polyclonal Antibody

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Catalog# ER1803-65

ITPR2 Rabbit Polyclonal Antibody

Application

  • Dot Blot

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ITPR2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human ITPR2 aa 1100-1450.

Species Reactivity

Human, Mouse, Rat

Validated Applications

Dot Blot, IF-Cell, IHC-P, FC

Target Molecular Weight

Predicted band size: 308 kDa

Positive Control

HepG2, HT-29, SiHa, rat kidney tissue, human colon tissue, human breast tissue, mouse liver tissue.

Conjugation

unconjugated

RRID

AB_3069260

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • Dot Blot

  • 1:2,000-1:5,000

  • IF-Cell

  • 1:50-1:100

  • IHC-P

  • 1:50 -1:200

  • FC

  • 1:50-1:100

Target

Function

The protein encoded by this gene belongs to the inositol 1,4,5-triphosphate receptor family, whose members are second messenger intracellular calcium release channels. These proteins mediate a rise in cytoplasmic calcium in response to receptor activated production of inositol triphosphate. Inositol triphosphate receptor-mediated signaling is involved in many processes including cell migration, cell division, smooth muscle contraction, and neuronal signaling. This protein is a type 2 receptor that consists of a cytoplasmic amino-terminus that binds inositol triphosphate, six membrane-spanning helices that contribute to the ion pore, and a short cytoplasmic carboxy-terminus. A mutation in this gene has been associated with anhidrosis, suggesting that intracellular calcium release mediated by this protein is required for eccrine sweat production.

Background References

1. Shi JL et al. High expression of inositol 1,4,5-trisphosphate receptor, type 2 (ITPR2) as a novel biomarker for worse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget 6(7):5299-5309(2015).

2. Zhang F et al. Genome-wide association study identifies ITPR2 as a susceptibility gene for Kashin-Beck disease in Han Chinese. Arthritis Rheumatol 67(1):176-181(2015).

Sequence Similarity

Belongs to the InsP3 receptor family.

Tissue Specificity

Isoform Short is found in skeletal muscle and heart.

Post-translational Modification

Phosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release.

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: Q14571 Human

SwissProt: Q9Z329 Mouse

SwissProt: P29995 Rat

Synonyms

4 antibody

5-trisphosphate receptor antibody

Inositol 1 4 5 trisphosphate receptor type 2 antibody

Inositol 1,4,5-trisphosphate receptor type 2 antibody

Inositol 145 trisphosphate receptor type 2 antibody

InsP3 R2 antibody

InsP3R2 antibody

IP3 R2 antibody

IP3 receptor antibody

IP3 receptor isoform 2 antibody

Expand

Images

  • Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.

    Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.

  • ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"